大麦紫黄素脱环氧化酶基因片段的克隆与分析  

Cloning and analysing of violaxanthin de-epoxidase gene of barley

作　　者：房蓓[1] 王少峡[2] 

机构地区：[1]天津医科大学生物医学工程系,天津300070 [2]天津师范大学生物系,天津300074

出　　处：《安徽大学学报（自然科学版）》2005年第2期100-102,共3页Journal of Anhui University(Natural Science Edition)

摘　　要：根据紫黄素脱环氧化酶基因(VDE)基因的保守序列设计引物,用RT-PCR的方法从大麦叶片总体RNA中克隆到VDE基因片段,测序结果显示其长度为 639bp,BLASTx比对显示与已发表的VDE基因有较高的同源性。Northern杂交表明VDE基因的表达水平成熟绿叶片中达到最高。With primers designed according to sequences of violaxanthin de-epoxidase (VDE) genes, a sequence of 639bp was amplified from barley total RNA by RT-PCR. The sequence has high homology with published VDE genes after sequencing and BLASTx aligning. Northern blot showed that the expression of VDE achieved maximum in mature green leaves.

关 键 词：酶基因 克隆 表达水平 RT—PcR 基因片段 RNA 测序 大麦 叶片 杂交 

分 类 号：N03[自然科学总论—科学技术哲学] S512.3[农业科学—作物学]