补肾清热毒方对cGVHD狼疮小鼠肾组织细胞凋亡的调节作用  

Regulatory Action of BushenqingreFang on Apoptosis of Nephridial Tissue in Mice with Lupus Nephritis Caused by cGVHD

作　　者：陈杨荣[1] 任文英[2] 江明[1] 阮诗玮[2] 陈壮威[2] 

机构地区：[1]福建中医学院,福州350003 [2]福建省人民医院,福州350004

出　　处：《中华中医药杂志》2005年第3期151-154,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省中医药重点课题 (编号 702)

摘　　要：目的: 探讨补肾清热毒方 (简称补清方) 对慢性移植物抗宿主病 (chronicgraft versus hostdisease, cGVHD) 狼疮样小鼠模型肾组织细胞Fas/FasL (Fasligand) 的调节作用。方法: 采用原位末端标记法 (TUNEL) 染色观察肾组织细胞凋亡; 免疫组化、蛋白印记 (westernblot) 和逆转录聚合酶链反应 (RT PCR) 技术检测Fas、FasL基因转录及蛋白表达情况。结果: 补清方可增加肾组织TUNEL阳性积分, 上调Fas、FasLmRNA和蛋白水平。结论: 补清方治疗狼疮性肾炎 (lupusnephritis, LN) 的作用可能是通过上调Fas、FasL, 促进细胞凋亡实现的。Objective: To study the regulatory action of BushenqingreFang on Fas/FasL in the cells of nephridial tissue in mice with lupus nephritis (LN) caused by chronic graft-versus-host disease (cGVHD). Methods: To observe the apoptosis in nephridial tissue by TUNEL and to measure the Fas/FasL genetic transcription and their protein expression by immunohistochemistry, western blot and RT-PCR techniques. Results: BushenqingreFang increased the positive score of TUNEL in nephridial tissue and up regulated mRNA expression and protein levels of Fas and FasL. Conclusion: The action of BushenqingreFang on LN may be conducted by its up regulation of Fas and FasL and its promotion of apoptosis.

关 键 词：补肾清热毒方 cGVHD狼疮 小鼠 肾组织细胞 细胞凋亡 调节作用 中医药疗法 

分 类 号：R259.9[医药卫生—中西医结合]