骨碎补总黄酮对成骨细胞体外培养作用的机制研究  

Study on Mechanism of Effects of Total Flavone of Rhizoma Drynariae on Osteoblasts Cultured in Vitro

作　　者：谢雁鸣[1] 秦林林[2] 邓文龙[3] 陈金标[2] 赵晋宁[1] 

机构地区：[1]中国中医研究院西苑医院,北京100091 [2]中日友好医院,北京100029 [3]四川中药研究所,成都640016

出　　处：《中华中医药杂志》2005年第3期161-162,共2页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家"九.五"科技攻关重大疾病课题 (96 906 09 04)

摘　　要：目的: 研究骨碎补总黄酮不同剂量组对成骨细胞体外培养的作用机理。方法: 购买UMA 106 01成骨细胞株进行成骨细胞培养, 观察碱性磷酸酶 (ALP) 活性和3H TdR掺入的情况。结果: 骨碎补总黄酮能提高用UMR 106细胞培养的细胞内ALP活性, 并且在不同时间 24、48、72h有相应变化, 有一定的量效、时效关系, 以 48h最为理想。骨碎补总黄酮对3H TdR的掺入 48h比 24h有明显增加, 有时效关系。结论: 骨碎补总黄酮对成骨细胞分化和增殖均有促进作用。Objective: To study the mechanism of effect of total flavone of rhizoma drynariae in groups with different dosage on osteoblasts cultured in vitro. Methods: To culture osteoblasts with UMA-106-01 osteoblast strain and to observe the activity of ALP and the endosmosis of 3H-TdR. Results: The total flavone of rhizoma drynariae increased the activity of ALP in cells cultured with UMR-106-01 strain. The ALP activities correspondingly changed in the 24h, 48h and 72h, which related to dosage effect and time effect. Among them, the activity in the 48h is the most ideal one. And the amount of endosmosis of the total flavone of rhizoma drynariae to 3H-TdR increased more remarkably in the 48h than that in the 24h and it also related to the time effect. Conclusion: The total flavone of rhizoma drynariae can promote the differentiation and multiplication of osteoblasts.

关 键 词：骨碎补总黄酮 成骨细胞 体外培养 作用机制 H-TdR掺入 碱性磷酸酶 

分 类 号：R285[医药卫生—中药学]