肿瘤坏死因子α基因多态性与矽肺的相关性  

Association of Tumor Necrosis Factor-α Gene Polymorphisms with Silicosis in Southwest Chinese Han Population

作　　者：王德军[1] 杨跃林[1] 夏庆杰[2] 兰亚佳[1] 王绵珍[1] 韩吉平[3] 孙敏[1] 

机构地区：[1]四川大学华西公共卫生学院,四川成都610041 [2]华西医院眼疾病分子遗传室 [3]攀枝花钢铁集团劳研所

出　　处：《职业卫生与病伤》2005年第1期1-4,共4页Occupational Health and Damage

摘　　要：目的　探讨肿瘤坏死因子α基因(tumornecrosisfactor -α,TNF -α)启动子区- 30 8和- 2 38位点多态性与中国西南地区汉族人群矽肺易感性的相关性。方法　分别选择96例矽肺患者和116名矽尘接触者为研究对象。采集外周静脉血,应用聚合酶链反应-限制性片段长度多态性分析检测其TNF-α基因多态性。结果　矽肺组中TNF -α- 30 8A、- 2 38A的分布频率显著高于正常矽尘接触组(P<0. 0 1) ;其中I期矽肺患者- 30 8A的分布频率与矽尘接触组差异无统计学意义(P >0 0 1) ,而II期、III期矽肺患者- 30 8A、- 2 38A的分布频率均远高于矽尘接触组(P <0 0 1)。调整混杂因素后,Lo gistic回归分析发现,矽肺组及各亚组和正常矽尘接触组- 30 8A、- 2 38A的分布频率差异均有统计学意义(P <0. 0 1)。结论　TNF -α- 30 8及- 2 38位点基因多态性与中国西南地区汉族人矽肺的发生及其严重程度有关。Objective To approach whether polymorphisms within the tumor necrosis factorαgene (TNFα) promoter region are associated with susceptibility to silicosis in Southwest Chinese Han population. Method 96 cases of silicosis and 116 silica exposed workers (being employed) were selected as subjects. 5 ml peripheral vein blood was drawn from each subject. By means of polymerase chain reaction restriction fragment length polymorphisms (PCRRFLP) techniques, TNFαgene polymorphisms of all subjects were analyzed. Result The frequencies of TNFα-308 A and-238 A were still associated with the presence of silicosis( P <0 01) Conclusion TNFα(-308 and-208) gene polymorphisms might be related to the occurrence of silicosis and the degree of severe pulmonary fibrosis in silicosis among the Han population of Southwest China.

关 键 词：肿瘤坏死因子Α 基因多态性 矽肺 职业卫生 汉族 

分 类 号：R135.2[医药卫生—劳动卫生]