用RAPD分析多年生黑麦草品种间遗传多样性  

Genetic Polymorphism of Ryegrass Cultivars Detected by RAPDs

作　　者：宁婷婷[1] 张再君[1] 金诚赞 朱英国[1] 

机构地区：[1]武汉大学生命科学学院植物发育生物学教育部重点实验室,武汉430072 [2]朝鲜国家科学院生物分院植物研究所

出　　处：《武汉植物学研究》2005年第1期27-31,共5页Journal of Wuhan Botanical Research

摘　　要：多年生黑麦草是世界上应用最广泛的冷季型草坪草兼牧草之一,具有建植快速,分蘖能力强,适口性良好等特点。为了评价其品种间的遗传多样性,选取了多年生黑麦草的14个草坪型品种和2个意大利黑麦草品种用RAPD进行遗传多样性分析,并且对每个品种的几项坪用特性进行了观察。61个引物共扩增出408条带,多态条带比率(PPB)为89.95%。聚类分析结果表明,多年生黑麦草品种之间的遗传多样性较低,相似系数分布于44.55%～95.69%之间。一年生黑麦草的2个品种TopOne和Avance没有单独聚成一类,而是与14个多年生黑麦草品种混合聚在一起,而且Avance与其他多年生黑麦草之间的相似系数较高。聚类结果与形态特征没有明确的对应关系。Perennial ryegrass is clearly one of the most important and widely used forage and turf grasses,exhibiting rapid establishment,good tillering ability and high acceptability to livestock.The genetic relationship of 16 ryegrass cultivars,including 2 accessions annual cultivars and 14 entries perennial cultivars,from different breeding company were analyzed using 61 RAPD mar-(kers),the morphological characters were also observed.61 RAPD primers generated 408 bands,of which 367 bands (89.95%) were polymorphism;It showed that the genetic polymorphism in these cultivars was low,the coefficient among the cultivars fell between 44.55%~95.69%.Dendrogram obtained using these molecular markers is not in agreement with their separated morphologic character.High correlation was not observed between the two annual ryegrass cultivars.

关 键 词：多年生黑麦草 意大利黑麦草 RAPD UPGMA 遗传多样性 

分 类 号：Q346.5[生物学—遗传学]