马传染性贫血病毒自然感染马LTR序列分析  

Comparison and analysis of the LTR sequences of equine infectious anemia virus wide-type strains

作　　者：魏丽丽[1,2] 王晓钧[1] 王盈[1,2] 李景鹏[3] 相文华[1] 沈荣显[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所 [2]东北农业大学生命科学院,黑龙江哈尔滨150030 [3]东北农业大学生命科学院

出　　处：《中国预防兽医学报》2005年第2期81-84,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助项目 (批准号 30 2 0 0 2 0 0 )

摘　　要：我国从 2 0世纪 70年代开始使用EIAV弱毒疫苗后 ,在全国范围内基本控制了马传贫的发生 ,我们从现地少数EIAV琼扩阳性马外周血中扩增并克隆了二株EIAV前病毒 5’LTR序列 ,并与国内外毒株 5’LTR序列进行了比较分析 ,发现中国EIAVLTR特有的特异性细胞转录因子结合基序 ,同时发现PEA2位点不是强毒特有的基序 ,在弱毒中亦发现该基序。Since using an ELAV attenuated vaccine in large scale in past 20 years in China,equine infectious anemia has been successfully controlled.However,a few horses were identified as sera positive against ELAV by AGID test.Here we amplified and cloned two provirus DNA 5'LTR sequences from PBMC of two horses.And compared the LTR sequences with that of different types of EIAV.According to the results of the comparison.we gound that the cell specificity of the transcription_factor in Chinese EIAV.PEA2 motif was been found in avirulent EIAV strains.

关 键 词：马传染性贫血病毒 长末端重复序列 序列测定 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]