rhBMP-2及其纳米微球缓释系统对成骨细胞钙结节影响的实验研究  

Effects of the recombinant human bone morphogenetic-2 polybutylcyanoacrylate nanoparticles on calcium nodules formed by cultured osteoblast

作　　者：侯锐[1] 毛天球[1] 杨耀武[1] 程晓兵[1] 陈富林[1] 吴炜[1] 

机构地区：[1]第四军医大学口腔医学院口腔颌面外科,陕西西安710032

出　　处：《临床口腔医学杂志》2005年第3期158-160,共3页Journal of Clinical Stomatology

基　　金：国家"973"项目资助课题 (G1 9990 5430 8)

摘　　要：目的 :观察rhBMP 2聚氰基丙烯酸正丁酯纳米微球缓释系统对成骨细胞钙结节的影响。方法 :培养诱导成骨细胞形成钙结节 ,并对其行特异性染色 ;在rhBMP 2纳米微球对钙结节作用后 ,采用扫描电镜观察其表面形态的改变 ,采用能谱仪分析比较其组成成分的改变。结果 :rhBMP 2纳米微球能明显促进钙结节形成 ,且rhBMP 2纳米微球作用后钙结节中钙磷含量显著高于单纯rhBMP 2组。结论 :rhBMP 2纳米微球缓释系统促进成骨的作用优于单纯rhBMP 2。Objective:To evaluate the effects of the rhBMP-2poly- butylcyanoacrylate nanoparticles sustained release system on calcium nodules formed by cultured osteoblasts.Method: Calcium nodules were acquired by cell culture technique and detected by specific staining. Scanning electron microscope and energy spectrum analysis were used to evaluate morphologic property and content of calcium in calcium nodules by adding the rhBMP-2 nanoparticles or only rhBMP-2.Result: The rhBMP-2 nanoparticles could enhance the formation of calcium nodules significantly, and statistical analysis showed that the content of calcium and phosphorus in calcium nodules were significantly higher in rhBMP-2 nanoparticles group than in rhBMP-2 group. Conclusion: The rhBMP-2 polybutylcyanoacrylate nanoparticles sustained release system has higher effects on enhancing osteogenesis than rhBMP-2.

关 键 词：重组人骨形成蛋白-2 聚氰基丙烯酸正丁酯 纳米微球 骨髓间充质干细胞 钙结节 

分 类 号：Q813.1[生物学—生物工程]