乳鼠心肌成纤维细胞培养方法的改进  

A modification of the model establishment for cell culture of neonatal rat myocardial fibroblasts

作　　者：陈勇兵[1] 陈如坤[2] 陈力[2] 吴明[2] 施立[1] 杨文涛[1] 钱永跃[1] 

机构地区：[1]苏州大学附属第二医院胸心外科,215004 [2]浙江大学附属第二医院胸心外科

出　　处：《江苏医药》2005年第3期189-190,F002,共3页Jiangsu Medical Journal

基　　金：苏州大学青年基金;浙江省医药卫生科学研究基金(编号2000A110)

摘　　要：　目的　建立乳鼠心肌成纤维细胞培养模型。方法　通过对经典的差速贴壁分离法稍作改进,采用每次贴壁60 min、两次差速贴壁分离法收集、培养心肌成纤维细胞。结果　形态学观察和免疫细胞化学染色鉴定显示,成功培养心肌成纤维细胞,纯度达 95%以上。结论　该研究成功建立乳鼠心肌成纤维细胞培养模型,为高纯度心肌成纤维细胞的获得和对其病理生理学的进一步研究打下良好基础。Objective To establish the model of cell culture of neonatal rat myocardial fibroblasts.Methods Neonatal rat myocardial fibroblasts were isolated and cultured with a modified technique of differential anchoring velocity for twice with 60 min each.Morphologic characters of those cells were observed with the inverted microscope.Immunocytochemical staining was used to evaluate and confirm the myocardial fibroblasts.Results The morphological observation and immunocytochemical staining used to confirm the myocardial fibroblasts showed that the purity of cells cultured was more than 95%.Conclusion The model of cell culture of neonatal rat myocardial fibroblasts was successfully established,which has laid good foundation for further research on myocardial fibroblasts. [

关 键 词：乳鼠 心肌成纤维细胞 细胞培养 免疫细胞化学染色 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]