核黄素操纵子在B.subtilis 24R7染色体上整合对核黄素合成的影响  

The Effect of Integration of Riboflavin Operon in B.subtilis 24R7 Chromosome on Riboflavin Production

作　　者：陈涛[1] 王靖宇[1] 班睿[1] 陈洵[1] 赵学明[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《无锡轻工大学学报（食品与生物技术）》2005年第1期6-10,共5页Journal of Wuxi University of Light Industry

基　　金：国家自然科学基金项目(20036010);国家973计划项目(2003CB716003)资助课题.

摘　　要：构建含有B.subtilis 24核黄素操纵子的整合型核黄素质粒pRB40、pRB61和pRB63,研究了核黄素操纵子分别在B.subtilis 24R7染色体的rib、amyE和thrC位点整合对核黄素合成的影响.结果表明,核黄素操纵子通过单交换重组方式整合在上述位点可使核黄素合成量增加14%～19%,而通过双交换重组方式整合在amyE或thrC位点可使核黄素合成量增加40%～44%.增强核黄素操纵子在B.subtilis 24R7染色体上的稳定性可以提高核黄素的合成量.Integrative rib plasmid pRB40、pRB61 and pRB63 containing the riboflavin operon of B.subtilis24 were constructed. The effect of integration of the operon in 24R7 chromosome at rib、amyE and thrC locus was studied. The results indicate that integration of the operon by a single-cross or double-cross event causes an increase of 14%~19% or 40%~44% in riboflavin production, respectively. Enhancing the stability of riboflavin operon integrated in the chromosome results in the improvement of riboflavin production.

关 键 词：核黄素 操纵子 染色体 影响 质粒 增加 位点 重组方式 整合 交换 

分 类 号：Q819[生物学—生物工程]