治疗性HIV-1重组DNA疫苗的构建与免疫效果评价  

Construction and Vaccination of Recombinant HIV-1 DNA Vaccine for Therapeutic Use

作　　者：宋英今[1] 金宁一[1] 张立树[1] 李子建[1] 金洪涛[1] 郎守民 

机构地区：[1]军事医学科学十一所全军基因工程重点实验室,长春130062 [2]吉林省延边州牧业局,延边133000

出　　处：《中国生物制品学杂志》2005年第2期126-128,共3页Chinese Journal of Biologicals

基　　金：受"863"项目 (2 0 0 3AA2 190 5 1)资助

摘　　要：目的　构建编码HIV- 1多表位抗原基因 (MEG)与p2 4基因嵌合的核酸疫苗 ,并评价其免疫效果。方法　将编码HIV -1多表位抗原基因 (MEG)与p2 4基因插入到真核表达载体pVAXI中 ,构建HIV- 1治疗性重组核酸疫苗质粒。通过ELISA试剂盒检测免疫恒河猴血清中HIV -1特异性抗体 ,并通过淋巴细胞转化实验检测HIV -1特异性T淋巴细胞增殖反应。结果　构建的重组核酸疫苗质粒pVAXI -MEGp2 4免疫恒河猴后 ,能有效地刺激产生T淋巴细胞特异性增殖反应 ,并诱导产生抗HIV -1特异性抗体。结论　所构建的重组核酸疫苗质粒能诱导机体产生免疫反应。Objective To construct the DNA vaccine contai ning HIV-1 multiple-epitope antigen gene and p24 gene and evaluate the immunologic al effect of the vaccine.Methods A recombinant DNA vaccine plas mid was constructed by inserting HIV-1 multiple-epitope antigen gene and p24 g en e into vector pVAXI.The HIV-1 specific antibodies in the sera of monkeys immuni zed with the vaccine were detected by ELISA.The HIV-1 specific T lymphocyte pro liferation was determined by lymphocyte transformation test. Results A recombinant vaccine plasmid pVAXI-MEGP24 was constructed .The expressed product stimulated the specific T lymphocyte proliferation effect ively and induced HIV-1 specific antibodies in monkeys. Conclusion The constructed DNA vaccine plasmid for therapeutic use indu ced strong immune response in monkeys. [

关 键 词：HIV-1 核酸疫苗质粒 治疗性 特异性抗体 MEG 恒河猴 多表位抗原 原基 免疫效果 ELISA试剂盒 

分 类 号：Q944.58[生物学—植物学] R512.91[医药卫生—内科学]