干扰素脂质体的制备及理化性质分析  

Preparation and Physiochemical Property of Interferon Liposome

作　　者：王玉梅[1] 郭桥[1] 李秀芝[1] 迟春萍[1] 王志武[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2005年第2期141-142,共2页Chinese Journal of Biologicals

摘　　要：目的　制备干扰素α2b脂质体 ,分析其理化性质。方法　采用旋转逆相蒸发法制备干扰素α2b脂质体 ,通过正交试验进行制备条件的优选 ,电镜观察其粒径大小 ,酶联免疫分析法测定包封率 ,并观察脂质体的稳定性。结果　干扰素α2b脂质体平均粒径为 98. 6nm ,最高包封率为 78 .2 3% ,脂质体存放于 4℃及 - 2 0℃稳定性良好。结论　本试验方法制备的脂质体包封率高 ,理化性质较稳定。Objective To prepare IFNα2b liposome and stu dy i ts physiochemical property.Methods Prepare interferon liposome by reverse rotary evaporation.Observe the grain size of the prepared IFNα2b lip osome by electron microscopy.Determine the envelopment rate by ELISA and observe the stability of liposome.Results The mean grain size of prepa red IFNα2b liposome was 98 6 nm,and the maximum envelopment rate was 78 23%.T he liposome showed good stability at both 4℃ and -20℃.Conclusion The prepared IFNα2b liposome showed high envelopment rate and stable physio chemical property. [

关 键 词：脂质体 干扰素Α2B 包封率 酶联免疫分析法 观察 制备条件 理化性质 平均粒径 粒径大小 正交试验 

分 类 号：Q545.1[生物学—生物化学] R944[医药卫生—药剂学]