银杏叶提取物对高脂胆固醇喂饲家兔血管神经酰胺含量和泡沫细胞凋亡的影响  

The Effects of Ginkgo Biloba Extract on Ceramide Content and Cell Apoptosis of Aorta in Rabbits Fed with High Cholesterol and Fat Diet

作　　者：黑子清[1] 吴伟康[2] 孙惠兰[2] 黄河清[2] 谭红梅[2] 

机构地区：[1]中山大学第三附属医院,广东广州510630 [2]中山大学医学院中西医结合研究所,广东广州510080

出　　处：《中国实验方剂学杂志》2005年第2期60-63,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金资助项目[2000](NO:30070930)

摘　　要：目的观察预防性应用银杏叶提取物对家兔实验性动脉粥样硬化的形成、血管壁神经酰胺量和泡沫细胞凋亡的影响,探讨银杏叶提取物抗AS的作用及可能机制。方法将24只新西兰家兔随机分为3组正常对照组、高胆固醇组、银杏叶提取物组。分别给予普通饲料(正常对照组)和高胆固醇饮食(其它2组)喂养;银杏叶提取物组另预防性给予银杏叶提出物;实验结束后,运用图象分析法测定主动脉内膜脂质斑块面积百分比;用高效薄层层析扫描分析血管组织神经酰胺的变化;用Tunel法检测血管组织泡沫细胞的凋亡指数。结果银杏叶提取物可明显缩小主动脉内膜脂质斑块面积,降低动脉组织神经酰胺浓度,减少血管壁泡沫细胞凋亡的数量。结论银杏叶提取物具有较好的抗AS作用,神经酰胺与动脉粥样硬化形成和泡沫细胞凋亡有关。Objective:To observe the effects of ginkgo biloba extract on anti-atherosclerosis,ceramide content of aorta and foam cell apoptosis in experimental rabbits and study the possible mechanism.Methods: 24 rabbits were randomly divided into three groups.Control group was fed with a normal diet;High cholesterol group was fed 1% cholesterol and 5% fat diet;ginkgo biloba extract group was fed 1% cholesterol and 5% fat diet plus ginkgo biloba extract 2.0g·kg^-1 ·d^-1 .At the end of study,the plaque area were measures by image analysis,the aorta ceramide were detected by HPTLC and foam cell apoptosis were evaluated by TUNEL method.Results: Ginkgo biloba extract could diminish lipid plaque area on the aortic endothelium,reduce the levels of aorta ceramide and the apoptosis index.Conclusions: Ginkgo biloba extract has inhibitory effect on AS.The possible mechanism may be related to the decreased concentration of ceramide in aorta by treatment of ginkgo biloba extract.

关 键 词：动脉粥样硬化 神经酰胺 银杏叶提取物 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]