脱细胞同种异体神经移植物桥接大鼠坐骨神经缺损的形态学观察  

Morphological study of acellular nerve allografts in sciatic nerve gap of rats

作　　者：孙晓红[1] 佟晓杰 张彩顺 王虹[1] 张旭 

机构地区：[1]中国医科大学附属第四医院神经内科,辽宁沈阳110032 [2]中国医科大学基础医学院人体解剖学教研室

出　　处：《中国医科大学学报》2005年第1期6-9,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30070775);辽宁省教育厅高校科研资助项目(20122177)

摘　　要：目的: 研究脱细胞处理的同种异体神经移植物桥接大鼠坐骨神经缺损后, 再生神经的形态学变化。方法: 光镜、电镜观察移植物内再生神经的超微结构变化, 免疫组织化学技术观察再生神经中S-100蛋白表达,并对再生神经的纤维数量、髓鞘厚度等进行统计学分析。结果: 实验组和自体神经移植对照组的移植段内见有由束膜和外膜包被的大量再生神经纤维, 两组比较再生神经的纤维数量、髓鞘厚度、有髓纤维占有的面积均无显著性差异; 两组移植段神经中的S-100阳性施万细胞数、形态和排列等方面也无明显差异。结论: 脱细胞同种异体神经同自体神经一样,对缺损的坐骨神经再生具有促进作用。Objective: To observe the morphological change of acellular nerve allografts in the sciatic nerve gap of rats. Methods:The morphological change was observed with light microscope and electron microscope,and the results were analyzed statistically.We used ABC immunohistochemical method to measure the expression of S-100 protein in the regenerated nerves. Results:There was no significant difference in number of the regenerated nerve fibers,the thickness of myelin sheath, and the expression of S-100 protein of the regenerated myelinated nerve between the experimental group and control group. Conclusion: The acellular nerve allografts could promote the proliferation of Schwann cells in vivo and have functions in bridging and promoting regeneration of injured peripheral nerve.

关 键 词：脱细胞同种异体神经移植物 神经再生 S-100蛋白 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]