Micro-propagation of Lepedium meyenii Walp. （Maca） by shoot culture  

作　　者：ChengHua YuLongjiang HuQiongyue SunYouping JinWenwen ChenShancai LiMaoteng 

机构地区：[1]CollegeofLifeScienceandTechnology,HuazhongUniversityofScienceandTechnology,Wuhan430074 [2]HunanAcademyofForestrySciences,Changsha410004,China

出　　处：《Agricultural Science & Technology》2004年第4期18-22,共5页农业科学与技术（英文版）

摘　　要：The callus induction and plant regeneration system for an important plant, Lepedium meyenii Walp., has been established. Calli were induced from cotyledons petioles of Lepedium meyenii Walp within 4 weeks in a modified MS medium supplemented with BA plus NAA. The highest percentage of callus formation (57. 1% ) was found on MS medium supplemented with 0. 5 mg l^-1 BA and 0. 5 mg l^-1 NAA. During subculture on the shoot formation medium, most of calli proliferated and 50% -60% formed shoots. About 66.7% of shoots formed into roots on 1/2 strength MS containing 0.5mg l^-1 IBA after 4 weeks in culture. Chromosome count confirmed the number of the regeneration Maca plantlet was the same as that of the native plant(8x=64) .For regeneration of plantlets, from seedling via primary callus production, a four-step process of organogenes is required about 16 weeks.

关 键 词：嫩芽 微繁殖 组织培养 药用植物 玛咖 外植体 培养基 

分 类 号：S567.2[农业科学—中草药栽培]