HPLC法测定穿心莲内酯滴丸中穿心莲内酯的含量  

Determination of the andrographolide in andrographolide pill by HPLC

作　　者：周家才 梁玉梅 周久培 

机构地区：[1]南京医药合肥天星有限公司,安徽合肥230061

出　　处：《安徽医药》2004年第4期284-285,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的　探讨测定穿心莲内酯滴丸中穿心莲内酯含量的方法。方法　采用HPLC法 ,色谱条件为 :Hyersil C1 8柱 ,甲醇 :水 (5 5∶4 5 )为流动相 ,流速 1 0ml·min-1 ,检测波长 2 2 5nm。结果　穿心莲内酯在 2 1 936～ 1 97 4 2 4mg·L-1 浓度范围内与峰面积呈良好的线性关系 (r=0 9999) ,平均回收率 99 6 1 % ,RSD =0 94 %。结论　HPLC法简便、准确、可控 。Aim HPLC method for the determination of andrographolide in andrographolide pill was established.Methods AHyper sill C 18 columm was used ,with the mobile phase of methanol water(55∶45).The flow rate was 1 ml·min -1 ,and the detection wavelength was 225 nm.Results The concentration of andrographolide was linearlyrelated to its peak area in the rang of 21 936～197 424 mg·L -1 ,and the average recovery was 99 61%,with RSD of 0 94%.Conclusion This method is simple and quick ,reproducible ,and can be used for the quantitative analysis.

关 键 词：穿心莲内酯 HPLC法 滴丸 含量 制方 色谱条件 RSD C18柱 平均回收率 浓度 

分 类 号：R286[医药卫生—中药学]