富勒醇对^(60)Coγ射线致小鼠损伤的防护作用  

Experimental studies of the radioprotective effect of fullerenol in ^(60)Co-γ radiation-injured mice

作　　者：蔡小青[1] 李宇国[1] 吴胜伟[1] 叶波[1] 冉铁成[1] 郭金学[1] 徐慧[1] 李文新[1] 

机构地区：[1]中国科学院上海应用物理研究所纳米生物医药研究室,上海201800

出　　处：《中华放射医学与防护杂志》2004年第6期520-522,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 (19975 0 66) ;中国科学院知识创新工程方向性资助项目

摘　　要：目的　研究C6 0 衍生物富勒醇对6 0 Coγ射线损伤的防护和恢复治疗作用及机制。方法分别在辐照前和辐照后给小鼠腹腔注射富勒醇 ,6 0 Coγ射线全身均匀照射 ,吸收剂量 5 0Gy ,测定小鼠体重、脾指数、胸腺指数、血象和SOD含量的变化 ;取小鼠脾脏并制成脾细胞悬液 ,应用流式细胞仪 (FCM)检测脾脏细胞的周期。结果　辐照损伤后小鼠体重减轻、脾指数和胸腺指数降低、血象中各项指标下降、脾脏细胞周期中S期所占的比例升高。富勒醇可促进上述指标不同程度的恢复 ,并使脾脏细胞周期中S期恢复正常。加速了脾脏细胞的分裂增殖 ,从而使脾细胞能较快的得到修复。结论　富勒醇对6 0 Coγ射线照射小鼠具有一定的防护和恢复治疗的作用。Objective To investigate the radioprotective effect of fullerenol. Methods ICR rats were given fullerenol intraperitoneally(i.p.) before and after whole-body 60Co γ-ray irradiation with 5.0 Gy. The change in body weight,index of spleen,index of thymus,peripheral blood counts and SOD activity were observed. Cell cycle of splenoeytes was detected with flow cytometry(FCM). Results Fullerenol showed significant radioprective effect on irradiated mice including increasing the weight of mice,protecting normal immune function of spleen and thymus,curing the decreasing white blood cell and platelet counts. Fullerenol also redused S phase ratio,promoted spleen cell division and proliferation. Conclusion Fullerenol has efficient capacity of radioprotection. The possible mechanism of the radioprotective effect of fullerenol was discussed in this paper.;

关 键 词：小鼠 ^60Coγ射线 富勒醇 脾脏细胞 脾指数 复治 脾细胞 治疗 体重 恢复 

分 类 号：R818[医药卫生—放射医学]