放射性核素内照射诱发大鼠外周血淋巴细胞HPRT基因突变  

Study on HPRT gene mutation of rat peripheral blood lymphocytes induced by internal exposure to radionuclides using cloning method

作　　者：崔凤梅[1] 赵经涌[1] 劳勤华[1] 王六一[1] 杨淑琴[1] 胡明江[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院放射毒理室,215007

出　　处：《中华放射医学与防护杂志》2004年第6期538-540,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国防科工委基金资助项目 (Y5 5 73 0 62 )

摘　　要：目的　用克隆法研究放射性核素内照射诱发的大鼠外周血淋巴细胞HPRT基因突变情况。方法　大鼠尾静脉注入晚期混合裂变产物 ,克隆法检测不同累积剂量和不同剂量率的内照射诱发的外周血淋巴细胞HPRT基因突变 ,并拟合剂量效应关系。结果　随着累积剂量和剂量率的增加 ,HPRT基因突变频率随之上升 ,其剂量效应关系符合线性平方模型。结论　克隆法是一种较敏感的检测辐射诱发HPRT基因突变的方法 ,HPRT基因突变可能作为辐射生物剂量计。Objective To study HPRT gene mutation in rat peripheral blood lymphocytes induced by internal exposure to radionuclides using cloning method. Methods The radionuclides were injected into the tail vein and HPRT gene mutation was detected using cloning method to study the relationship of dose vs.response and of doserate vs.response. Results The HPRT gene mutation frequecy rose with the increase of accumulative dose and the doserate,both relationships could be described by linearquadratic models. Conclusion Cloning method is relatively sensitive to detect HPRT gene mutation induced by radiation and HPRT gene mutation may be used as a radiobiological dosimeter.;

关 键 词：HPRT基因突变 外周血淋巴细胞 辐射诱发 内照射 大鼠 剂量效应关系 放射性核素 累积 克隆 合剂 

分 类 号：R817[医药卫生—影像医学与核医学]