安美汀、红霉素对铜绿假单胞菌菌膜的作用研究  

Effects of augmentin and erythromycin on pseudomonas aeruginosa biofilm

作　　者：夏前明[1] 肖贞良[1] 李鸿雁[1] 李福祥[1] 全燕[1] 金成勇[1] 陈琰[2] 

机构地区：[1]成都军区总医院呼吸科,四川成都610083 [2]第三军医大学新桥医院,重庆400037

出　　处：《西南国防医药》2004年第2期122-124,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的 :观察安美汀、红霉素在体外对铜绿假单胞菌菌膜的作用。方法 ;将聚乙烯膜和绿脓杆菌置LB培养基中培养 4 8h ,扫描电镜观察有菌膜形成时取出聚乙烯膜 ,漂洗后分别放入含有不同浓度安美汀和红霉素的LB培养基中 ,2 4h后观察菌膜情况 ,并将培养液接种至LB琼脂平板观察是否有菌落形成。结果 :聚乙烯膜和铜绿假单胞菌共培养 4 8h ,扫描电镜观察到有菌膜形成 ;浓度为 10 0、30 0和 5 0 0 μg/ml的安美汀和浓度为 5 0、10 0和 30 0 μg/ml的红霉素作用 2 4h,聚乙烯膜上的铜绿假单胞菌菌膜均消失 ,培养液接种至LB琼脂平板无菌落形成。结论 :聚乙烯膜和铜绿假单胞菌共培养 4 8h可成功制作铜绿假单胞菌菌膜的体外模型 ;Objective:To observe the effects of augmentin and erythromycin on pseudomonas aeruginosa biofilm in vitro.Methods:polyethylene film and pseudomonas aeruginosa were co-cultured in LB medium for 48 hours until pseudomonas aeruginosa biofilm formed on polyethylene film.After that ,polythylene films were washed and transferred to LB medium containing different concentration of Augmentin and Erythromycin for further culture.polyethylene films were examined by scanning electric microscope to confirm if there were pseudomonas aeruginosa biofilms on it 24 hous later and LB media were vaccinated in LB agar plate to observe if pseudomonas aeruginosa were still alive in the cultured medium.Results:pseudomonas aeruginosa biofilm was formed on polyethylene film under scanning electric microscope after pseudomonas aeruginosa and polyethylene film were co-cultured in LB medium for 48 hours,howe ver,the biofilm disappeared after polyethylene film was cultured in media containing 100μg/ml,300μg/ml,500μg/ml of augmentin and 50μg/ml,100μg/ml,300μg/ml of Erythromycin.Conclusion:pseudomonas aeruginosa biofilm can be formed after polyethylene film and pseudomonas aeruginosa were co-cultured in LB medium for 48 hours.Adequate high concentration of augmentin and erythromycin can kill pseudomonas aeruginosa covered with biofilm in vitro.

关 键 词：菌膜 铜绿假单胞菌 安美汀 红霉素 

分 类 号：R54[医药卫生—心血管疾病]