日本血吸虫脂肪酸结合蛋白DNA联合白细胞介素-12的免疫保护作用  

The Immunoprotective Effect of Co-inoculation of Schistosoma Japonicum Fatty Acid Binding Protein (Sj14FABP)DNA and IL-12 in the Mouse

作　　者：朱晓华[1] 石佑恩[1] 

机构地区：[1]华中科技大学同济医学院寄生虫学教研室,武汉430030

出　　处：《医药导报》2005年第4期268-270,共3页Herald of Medicine

基　　金：国家高技术研究发展计划 ( 863计划 )资助项目(基金编号: 2004AA2Z3212)

摘　　要：目的　探讨白细胞介素-12 (IL-12)对日本血吸虫脂肪酸结合蛋白 (Sj14FABP)DNA疫苗的辅佐作用。方法　大量制备pVIVO2、pVIVO2 IL-12、pVIVO2 Sj14FABP质粒DNA。48只雄性Balb/c小鼠随机分为A、B、C、D组,每组各 12只,每只小鼠于免疫前 1d在右后腿股四头肌肌内注射 0 75%盐酸布比卡因 30μL。A组给予 0 9%氯化钠注射液 100μL,im;B组给予pVIVO2质粒DNA 100μg,im; C组给予pVIVO2 Sj14FABP质粒DNA 100μg,im;D组给予pVIVO2 Sj14FABP质粒DNA50μg和pVIVO2 IL 12质粒DNA50μg,im。免疫 30d后,每只小鼠以 ( 40±2 )条尾蚴感染,感染 45d后,计数成虫及肝内虫卵;用ELISA法检测小鼠血清中IgG抗体水平。结果　C、D组的减虫率分别为24 11%和 38 83%,减卵率为 27 20%和 40 27%,差异有显著性(P<0. 05);免疫 30d后小鼠血清IgG水平无明显升高,各组间差异无显著性 (P>0 05)。结论　pVIVO2 Sj14FABP能够诱导小鼠产生部分抗血吸虫感染的保护力,IL-12是一种有效的血吸虫病DNA疫苗佐剂。Objective To probe into the adjuvant effect of interleukin 12(IL 12) in the immunization of mice with Schistosoma japonicum fatty acid binding protein (Sj14FABP)DNA vaccine. Methods pVIVO2,pVIVO2 IL 12,pVIVO2 Sj14FABP plasmid DNAs were prepared in large amounts. 48 male Balb/c mice were randomly divided into 4 equal groups : A,B,C and D. Mice of all the 4 groups were given each an I.M. injection of 30 μL of 0.75% bupivacaine hydrochloride into the quadriceps femoris . One day later, mice of group A ,B,C and D were given each an I.M. injection of 100 μL of 0.9% sodium chloride solution, 100 μg of pVIVO2 plasmid DNA, 100 μg of pVIVO2 Sj14FABP plasmid DNA and 50 μg of pVIVO2 Sj14FABP plasmid DNA as well as 50μg of pVIVO2 IL 12 plasmid DNA ,respectively. 30 days thereafter, each mouse of all the 4 groups was challenged with (40±2 ) Schistosoma japonicum cercariae. 45 days after the challenge, all of the animals were sacrificed for counting the number of the recovered adult worms and the eggs of the parasites in the liver. The serum levels of IgG antibodies were assayed with ELISA. Results The worm reduction rates of group C and D were 24.11% and 38.83% ,respectively, meanwhile, the egg reduction rates in the liver of group C and D were 27.20% and 40.27% , respectively. The difference between groups D and group C were significant( P <0.05). 30 days after the immunization, the titer of IgG in mice of all the 4 groups did not increase dramatically,and the differences of the IgG levels were not significant among the 4 groups ( P >0.05). Conclusion Sj14FABP DNA vaccine was shown to induce a partial protective immunity against Schistosoma japonicum infection in Balb/c mice, and IL 12 was found to be a good adjuvant of Schistosoma DNA vaccine.

关 键 词：日本血吸虫脂肪酸结合蛋白 白细胞介素-12 DNA疫苗 佐剂 

分 类 号：R978.6[医药卫生—药品] R967[医药卫生—药学]