皱瘤海鞘有效部位体外抗乙型肝炎病毒表面抗原和e抗原的研究  

The Inhibitory Effect of the Active Principle of Styelec Plicata on HBsAg and HBeAg in Vitro

作　　者：胡文军[1] 万新祥[1] 

机构地区：[1]第一军医大学分校药剂教研室,广州510315

出　　处：《医药导报》2005年第4期276-277,共2页Herald of Medicine

基　　金：广州市科技攻关项目 (基金编号: 2002Z3 E0184)

摘　　要：　目的　研究皱瘤海鞘有效部位的体外抗乙型肝炎病毒 (HBV)的作用。方法　从新鲜皱瘤海鞘中提取分离皱瘤海鞘有效部位,应用HBV转染的 2 2 15细胞株,测定培养上清液中表面抗原 (HBsAg)、e抗原 (HbeAg)滴度,作为药物抗HBV效果的评价指标;并通过四氮唑(MTT)法测定皱瘤海鞘有效部位对细胞生长的半数毒性浓度,测定药物治疗指数。结果　皱瘤海鞘有效部位对HBsAg、HBeAg的分泌均有较明显的抑制作用,对细胞的毒性低,其治疗指数均>10。结论　皱瘤海鞘有效部位体外具有明显的抗HBV作用。Objective To study the anti HBV effect of the active principle of Styelec plicata in vitro. Methods The active principle of Styelec plicata was extracted and separated from the fresh organism. HBV transfected cells of the 2.2.15 cell line were coincubated with the active principle in different concentrations(64,32,16,8,4,2,1 mg·mL 1 ) in 96 well plates for 12 days. The titers of HBsAg and HBeAg in the supernatants of the culture medium were then determined and served as indices for assessing the anti HBV effect of the drug. The median toxic dose(TD 50 ) of the active principle for the cells were assayed with the MTT test. The therapeutic indices of the drug were also determined. Results The active principle of Styelec plicate was shown to have a relatively prominent inhibitory effect on the secretion of both HBsAg and HBeAg. The toxicity of the principle to the cells was low and its therapeutic indices were all greater than 10. Conclusion The active principle of Styelec plicata was shown to have an evident anti HBV effect in vitro.

关 键 词：皱瘤海鞘 2.2.15细胞株 乙肝表面抗原 乙肝E抗原 

分 类 号：R282.74[医药卫生—中药学] R512.62[医药卫生—中医学]