ClO^--鲁米诺流动注射-抑制化学发光法测定银杏黄酮  

Determination of flavonoids of Ginkgo biloba by ClO^--Luminol flow injection with inhibited chemiluminescence

作　　者：上官小东[1] 王福生[1] 郎惠云[2] 

机构地区：[1]宝鸡职业技术学院师范部,宝鸡721400 [2]西北大学化学系,西安710069

出　　处：《分析试验室》2005年第3期23-26,共4页Chinese Journal of Analysis Laboratory

摘　　要：基于在碱性条件下,银杏黄酮对ClO- 鲁米诺体系有显著的抑制作用,结合反相流动注射技术,建立了流动注射 抑制化学发光测定银杏提取物中的银杏黄酮。银杏黄酮质量浓度在0.4～14.0μg mL范围与相对发光强度呈线性关系,检出限为0.06μg mL,采样频率为120次 h,对10.0μg mL的银杏黄酮平行测定11次,其RSD为1.2%,回收率为98.0%～104%。该方法可用于银杏黄酮制剂的测定。A reversed flow injection with inhibited chemiluminescence method for the determination of flavonoids of Ginkgo biloba was firstly discussed. It was found that the CL intensity of the ClO^-- Luminol system could be inhibited in the presence of flavonoids of Ginkgo biloba. The decrease of CL emission was correlated with flavonoids of Ginkgo biloba concentration in the range from 0.4 to 14 μg/mL, the detection limit was 0.06 μg/mL and the relative standard deviation was 1.2% (c=10.0 μg/mL, n=11), a sampling frequency of 120/h was attained. The method was applied to the determination of flavonoids of Ginkgo biloba with recoveries of 98%～104%.

关 键 词：银杏黄酮 流动注射 抑制化学发光 

分 类 号：O657.32[理学—分析化学]