藉L-半胱氨酸包覆的ZnS纳米粒子的共振光散射定量分析蛋白质  

Application of L-cysteine-capped nano-ZnS as a new fluorescence probe for the determination of proteins by resonance light scattering technique

作　　者：张萍[1] 卓淑娟[1] 陈金龙[1] 汪乐余[1] 朱昌青[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,芜湖241000

出　　处：《分析试验室》2005年第3期86-90,共5页Chinese Journal of Analysis Laboratory

基　　金：安徽省自然科学基金资助项目(2001KJ114ZD)

摘　　要：合成和表征了功能性L 半胱氨酸包覆的ZnS纳米粒子。在pH5.12的NaAc HAc溶液介质中,L 半胱氨酸包覆ZnS纳米粒子于波长308.0nm处出现共振光散射峰。一定量蛋白质的加入能明显增强体系的共振光散射,且峰强度增加值与蛋白质浓度间存在良好线性关系,据此建立了一种灵敏的测定微量蛋白质的方法。用L 半胱氨酸包覆ZnS纳米粒子作为探针,不仅克服了有机染料可能出现的光漂白等缺点,而且本身不具毒性。该法用于人血清试样中总蛋白的测定,其结果与临床数据一致。L-cysteine-capped nano-ZnS particles (ZnS-Cys) as fluorescence probes have been successfully synthesized. The resonance light scattering (RLS) of ZnS-Cys in pH 5.12 NaAc-HAc solution is greatly enhanced by proteins with the scattering peak at 308.0 nm. The enhanced RLS intensity is in proportion to the concentrations of proteins. A sensitive RLS method for determining micro-level proteins has been developed. ZnS-Cys are non-toxic and stable, permitting highly resistant to photobleaching. The method has been applied to determine total protein in human serum samples with satisfactory results.

关 键 词：共振光散射 L-半胱氨酸包覆ZnS纳米 蛋白质 

分 类 号：O657.31[理学—分析化学]