人类新基因WDR70的克隆及初步研究  

The Clone of Human Novel Gene WDR 70 and Its Tentative Study

作　　者：单宏爽[1] 郭丽丽[1] 慈宏亮[1] 李亦平[1] 

机构地区：[1]北京师范大学生命科学学院生物医学研究所,北京100875

出　　处：《微生物学杂志》2005年第1期11-15,共5页Journal of Microbiology

摘　　要：运用基于基因组数据库的特定基因同源新基因的克隆策略得到一个人类新基因WDR70 ,该基因编码一个包含 12个WD4 0结构域的蛋白。WDR70的cDNA序列长 2 2 6 6bp ,预测编码蛋白含 6 30个氨基酸 ,理论分子量为 70× 10 3 u ,染色体定位为 17p13.1。以小鼠胚胎为模型进行整体原位杂交 ,结果显示WDR70基因在 8.5d小鼠胚胎中没有表达 ,而在 9.5d和 10 .5d的小鼠胚胎的脑部有特异表达。由此推断该基因对胚胎期脑部的发育有重要的影响。A human novel gene WDR70 is obtained with the cloning strategy of novel homologous gene,which is a specific gene based on genome database. It encodes a protein of 12 WD40 domain. The cDNA length of WDR70 is \{2266\} bp, and the putative protein encoded by this gene is 630 amino acid residues with a theoretical molecular weight of 70000 daltons. The WDR70 is mapped to chromosome at 17p13.1. The result of whole-mount in situ hybridization showed that it is strongly expressed in the brain of embryo in day 9.5 and 10.5 mice although it isn't expressed in the embryo in day 8.5. Thus, it is concluded that this new gene has great influence upon the development of the embryo brain.

关 键 词：同源分析 WD40结构域 生物信息学 整体原位杂交 

分 类 号：Q75[生物学—分子生物学]