SPE-HPLC法测定菊延保康颗粒中蒙花苷的含量  

SPE-HPLC Determination of Buddleoside in Juyan Baokang Granules

作　　者：陈蕾[1] 刘文英[2] 朱霁虹[1] 李永庆[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]中国药科大学,南京210009

出　　处：《药物分析杂志》2005年第3期270-273,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定菊延保康颗粒中蒙花苷含量的方法。方法:选用Oasis HLB固相萃取小柱对样品进行纯化。采用C_(18)色谱柱(4.6mm×250 mm,5μm),以甲醇-0.8%磷酸(1:1)为流动相,流速为1 mL·min^(-1),检测波长为326 nm。结果:蒙花苷进样量在0.0432—2.16μg的范围内与峰面积呈良好的线性关系,r为0.9999,平均回收率为100.4%。结论:本方法操作简便快速,结果准确可靠。Objective:To establish a method for the determination of buddleoside in Juyan Baokang granules by SPE-HPLC.Methods:Samples were purified by Oasis HLB SPE column.The HPLC column was C_(18) (4.6 mm×250mm,5μm);the mobile phase was methanol-0.8%phosphoric acid(1:1) ;the flow rate was 1 mL·min^(-1); the detection wavelength was 326nm.Results:The calibration curve of buddleoside was linear in the range of 0.0432 -2.16μg(r=0.9999).The average recovery (n=6) was 100.4%.Conclusion:The method is rapid, accurate and reliable.

关 键 词：SPE—HPLC法 含量 C18色谱柱 检测波长 纯化 固相萃取 流动相 HLB 颗粒 磷酸 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]