人血白蛋白对鲎试验干扰作用的定量分析  

Quantitative Analysis of Interference on the Limulus Test from Human Albumin

作　　者：胡冠时[1] 魏纪鲁[1] 贡立青[2] 赵海山[2] 

机构地区：[1]济南军区总医院,济南250031 [2]天津大学无线电厂,天津300072

出　　处：《药物分析杂志》2005年第3期345-348,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:分析不同浓度人血白蛋白注射液对鲎试验生化反应干扰程度的量变关系。方法:将人血白蛋白注射液稀释成不同浓度,并加入已知的标准内毒素,用动态浊度标准曲线法定量检测所加内毒素的回收率,测绘稀释-回收率曲线,计算不同浓度人血白蛋白注射液的干扰作用。结果:标准曲线方程Y=0.486X+2.8251 r=0.9977,标准曲线平均回收率为(100.1±6.04)%。精密度测定RSD 8.0%-15.0%。人血白蛋白注射液的稀释-回收率曲线呈两个时相,快速相和慢速相。结论:内毒素的回收率随着白蛋白浓度的减少而增加,人血白蛋白注射液稀释8倍时,回收率已达到87%,基本上已无干扰。Objective: Quantitative analysis of the interference on Iimulus test with different concentration of human albumin. Methods: Add a known standard endotoxin (NSE) to human albumin injection. The mixture was diluted in series. The endotoxin of series diluted mixtures was measured by a kinetic turbidimetric method. The recovery of NSE in the mixtures was analyzed,and a curve of human albumin concentration and endotoxin recovery was made. The interference of human albumin concentration on the accuracy of endotoxin detected by the Iimulus test was analyzed. Results:The standard endotoxin curve was made by the series of NSE solutions. The relationship of concentration and turbidity was described as following formulas; Y=0. 4862X + 2. 8251 r=0. 9977. The recovery was (100. 1±6. 04) %. The daily variant of the precision CV(%) was from 8.0% to 15.0%. The recovery curve of NSE in the series diluted human albumin mixtures showed diphase,consisting of a rapid-phase and a slow-phase. Conclusion:The recovery of endotoxin was increased with the decrease of human albumin concentration. When human albumin concentration was diluted to eighth,the interference became no significant and the endotoxin recovery reached up to 87%.

关 键 词：人血白蛋白 注射液 鲎试验 内毒素 干扰作用 标准曲线 定量检测 稀释 浓度 平均回收率 

分 类 号：R927[医药卫生—药学] R392-33