Salt tolerance conferred by over-expression of OsNHX1 gene in Poplar 84K  

作　　者：WANGShuyao CHENQijun WANGWenlong WANGXuechen LUMengzhu 

机构地区：[1]DepartmentofLifeScience,HunanUniversityofArtsandScience,Changde415000,China//StateKeyLaboratoryofPlantPhysiologyandBiochemistry,CollegeofBiologicalSciences,ChinaAgriculturalUniversity,Beijing100094,China [2]StateKeyLaboratoryofPlantPhysiologyandBiochemistry,CollegeofBiologicalSciences,ChinaAgriculturalUniversity,Beijing100094,China [3]DepartmentofLifeScience,HunanUniversityofArtsandScience,Changde415000,China [4]ResearchInstituteofForestry,ChineseAcademyofForestry,Beijing100091,China

出　　处：《Chinese Science Bulletin》2005年第3期224-228,共5页

基　　金：This work was supported by the National Basic Research Program of China(Grant Nos.G1999011704 and 2003CB114307).

摘　　要：OsNHX1 gene (Na+/H+ antiporter gene of Oryza sativa L.) was introduced into Poplar 84K with Agrobacte- rium tumefaciens-mediated transformation. PCR, Southern and Northern blot analysis showed that OsNHX1 gene was incorporated successfully into the genome of Poplar 84K and expressed in these transgenic plants. Salt tolerance test showed that three lines of transgenic plants grew normally in the presence of 200 mmol/L NaCl, while the Na+ content in the leaves of the transgenic plants grown at 200 mmol/L NaCl was significantly higher than that in plants grown at 0 mmol/L NaCl. The osmotic potential in the transgenic plants with high salinity treatment was lower than that of control plants. Our results demonstrate the potential use of these transgenic plants for agricultural use in saline soils.OsNHXl gene (Na^+/H^+ antiporter gene of Oryza sativa L.) was introduced intoPoplar 84K with Agrobacte-rium timefaciens-mediated transformation. PCR, Southern and Northern blotanalysis showed that OsNHXl gene was incorporated successfully into the genome of Poplar 84K andexpressed in these transgenic plants. Salt tolerance test showed that three lines of transgenicplants grew normally in the presence of 200 mmol/L NaCl, while the Na+ content in the leaves of thetransgenic plants grown at 200 mmol/L NaCl was significantly higher than that in plants grown at 0mmol/L NaCl. The osmotic potential in the transgenic plants with high salinity treatment was lowerthan that of control plants. Our results demonstrate the potential use of these transgenic plantsfor agricultural use in saline soils.

关 键 词：白杨 OsNHX1基因 耐盐性 分析方法 氨基酸 

分 类 号：Q949.733[生物学—植物学]