热休克反应对HMGB1表达及LPS诱导的HMGB1释放的影响  

Effects of Heat Shock Response on the Expression and LPS-induced Release of HMGB1

作　　者：唐道林[1] 石永忠[1] 蒋磊[1] 王慷慨[1] 肖卫民[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室,湖南长沙410008

出　　处：《医学临床研究》2005年第3期289-292,共4页Journal of Clinical Research

基　　金：国家自然科学基金重点项目(30330280);国家自然科学基金(30371382;30300177)

摘　　要：【目的】观察热休克反应对高迁移率族蛋白 1(HMGB1)表达及内毒素(LPS)诱导的 HMGB1 释放和移位的影响。【方法】用500 ng/ml LPS处理小鼠RA)264.7巨噬细胞20 h,)estern blot分析HMGB1在胞核及其培养上清中的改变;热休克模型通过RA)264.7 细胞置 42.5℃水浴锅中孵育 1 h,然后在 37℃恢复12 h后制备;通过)estern blot分析,观察热休克反应对HMGB1表达及LPS诱导的HMGB1释放及其移位的影响。【结果】HMGB1蛋白表达水平在热休克反应 4 h后减少,8 h后明显减少,12 h后逐步恢复至正常水平;RA)264.7细胞受LPS处理20 h后,细胞培养基中HMGB1的水平明显增加,胞核HMGB1含量减少,而热休克预处理抑制了LPS诱导的HMGB1释放及胞核HMGB1减少。【结论】热休克能影响HMGB1的基因表达;热休克反应抑制LPS诱导的HMGB1释放及其移位。Objectives]To study the effects of heat shock response (HSR) on high mobility group box 1 protein (HMGB1) expression and LPS(lipopolysaccharide)-induced release and translocation of HMGB1.Murine macrophage-like RAW 264.7 cells were stimulated with 500 ng/ml LPS for 20 h. The changes of HMGB1 in the nucleus and cell conditioned medium were analyzed by western blot. HSR was performed by incubating RAW 264.7 cells at 42.5°C for 1 h then recovery at 37℃ for 12 h. The effects of HSR on expression and release of HMGB1 were observed by western blot analysis.HMGB1 protein levels decreased at 4 h and significantly reduced at 8 h and gradually restored to a normal level at 12 h after heat shock stress. The levels of HMGB1 in cell conditioned medium increased and the nuclear HMGB1 levels decreased after RAW 264.7 cells were stimulated with LPS for 20 h. However, heat shock pretreatment significantly inhibited LPS-induced HMGB1 release and translocation from the nucleus to the cytoplasm in RAW 264.7 cells.[Conclusion]Heat shock response significantly inhibits gene expression and LPS-induced release and translocation of HMGB1.

关 键 词：热休克反应 HMGB1 LPS诱导 表达 RAW264.7 影响 观察 细胞 释放 水平 

分 类 号：R631[医药卫生—外科学] R394[医药卫生—临床医学]