蛇毒蛋白C激活剂的活化蛋白C特性及影响因素的探讨  

Research on the Protein C-Activating Property and Influencial Factors of Protein C Activator Isolated from the Venom of Agkistrodon halys

作　　者：侯力强[1] 赵路宁[1] 江中喜[2] 黄韶玲[1] 何泉华[1] 孔天翰[1] 管锦霞[1] 

机构地区：[1]广州医学院蛇毒研究所,510182 [2]广州医学院第二附属医院,510260

出　　处：《中药材》2005年第3期198-201,共4页Journal of Chinese Medicinal Materials

基　　金：广东省医学科学技术研究基金 (B2000061 );广州市教委科研项目基金资助

摘　　要：目的:研究蛇毒蛋白C激活剂(PCA-SV)活化蛋白C的特性,探讨可能影响PCA-SV作用的实验条件.方法:分别采用APTT法和CBS02.46发色底物法,研究PCA-SV的作用特点,探讨PCA-SV在不同实验条件下的抗凝活性及酰胺酶活性.结果:PCA-SV是蛋白C特异性激活剂,可特异性地作用于蛋白C,使后者表现抗凝和酰胺酶活性.PCA-SV的最适pH值为6.0～7.0,最适温度为37～40℃,肝素、枸橼酸钠等抗凝剂及Na+、K+、EDTA等不影响其活性.结论:PCA-SV是一种效价较高、特异性强、影响因素少的蛋白C活化剂.Objective:To investigate the protein C activating property and experimental conditions that may affect the activities of protein C activator (PCA SV) Methods:PCA SV's functional characteristic,possible anticoagulant and amidase activities were measured by method of APTT and that of specific chromogenic substrate respectively in various experimental conditions Results:PCA SV can specifically act on protein C The pH optimum and temperature optimum for the action of PCA SV were 6 0~7 0 and 37~40℃ respectively. The anticoagulants (heparin, sodium citrate) had no effect on the activities Conclusion:PCA SV is a new protein C activating reagent that has high potency and specificity

关 键 词：蛇毒 蛋白C激活剂 活化蛋白C 抗凝剂 酰胺酶 酶活性 

分 类 号：R996.3[医药卫生—毒理学] R96[医药卫生—药学]