配体形状对多吡啶铜(Ⅱ)配合物与DNA作用的影响  

Effects of Ligands Shape on DNA-Binding of Copper(II) Polypyridyl Com plexes

作　　者：张黔玲[1] 刘剑洪[1] 张培新[1] 任祥忠[1] 李翠华[1] 洪伟良[1] 刘建忠[2] 计亮年[2] 

机构地区：[1]深圳大学师范学院化学与生物学系,深圳518060 [2]中山大学教育部基因工程重点实验室,广州510275

出　　处：《无机化学学报》2005年第3期344-348,共5页Chinese Journal of Inorganic Chemistry

基　　金：广东省自然科学基金博士启动基金(No.04300882);深圳市科技计划资助项目(No.200328)

摘　　要：合成了一系列含有平面配体的Cu髤多吡啶配合物[Cu(IP)2]2+、[Cu(PIP)2]2+、[Cu(DPPZ)2]2+和[Cu(HPIP)2]2+,用吸收光谱、CD光谱和粘度等方法研究了这些配合物与小牛胸腺DNA的作用。结果表明配体上的取代基及配体的平面性对这些四面体配合物与DNA的结合强弱产生一定的影响。[Cu(DPPZ)2]2+与DNA的结合较强,而[Cu(HPIP)2]2+与DNA的结合较弱。CD光谱显示配合物[Cu(DPPZ)2]2+、[Cu(PIP)2]2+和[Cu(HPIP)2]2+的加入会导致DNA的CD光谱减弱。而[Cu(IP)2]2+的加入则会使DNA的CD光谱增强。同时,[Cu(IP)2]2+与DNA结合后,还会引起一定程度的DNA构型转换,即DNA从B型转换成Z型。A series of copper(II) polypyridyl complexes 2+, 2+ , 2+ and 2+ were prepared. Their DNA-binding properties we re studied by absorption spectra, CD spectra and viscosity measurements. The res ults indicated that the substituents and the planarity of ligands would have eff ects on binding affinity of these complexes to DNA. 2+ showed stronge r binding affinity for DNA, while 2+ showed relatively weaker binding affinity for DNA. CD spectra indicated that the addition of 2+, 2+ and 2+ decreased the CD intensity, while the addition of 2+ increased the CD intensity. In addition, binding of 2+ to DNA induced the configuration transition of DNA from B to Z.

关 键 词：DNA 配体 CD光谱 影响 作用 减弱 体形 铜(Ⅱ)配合物 吡啶 取代基 

分 类 号：O614.612[理学—无机化学] R972[理学—化学]