毛新杨×毛白杨叶片表型和春季萌芽时间QTL分析(英文)  

QTL Analysis of Leaf Morphology and Spring Bud Flush in (Populus tomentosa×P. bolleana)×P. tomentosa

作　　者：张德强[1] 张志毅[1] 杨凯[2] 李百炼[3] 

机构地区：[1]北京林业大学毛白杨研究所,北京100083 [2]中国农业科学院品种资源研究所,北京100081 [3]美国北卡罗莱纳州立大学林学系,北卡罗莱纳州nc276958203

出　　处：《林业科学》2005年第1期42-48,共7页Scientia Silvae Sinicae

基　　金：ThisworkwassupportedbyagrantfromtheProjectofNationalNaturalScienceFoundationofChina (3 0 170 780 ) .

摘　　要：以毛新杨无性系TB0 1×毛白杨无性系LM5 0的回交子代 12 0株个体为作图群体 ,对控制叶片表型性状如叶长、叶宽、叶面积和叶柄长以及春季萌芽时间共 5个性状的数量性状位点 (quantitativetraitloci,QTLs)进行分析。运用AFLP标记技术结合拟测交作图策略构建了含有 393个AFLP标记的毛白杨及毛新杨的遗传连锁框架图。毛白杨遗传图上共含有 2 4 7个AFLP标记位点 ,连锁位点覆盖毛白杨基因组总长约 32 6 5 1cM ,而毛新杨遗传连锁图上共含有 14 6个标记位点 ,连锁位点覆盖毛新杨基因组总长约 1992cM ,这些连锁图的每一连锁群上含有的标记数为 4～ 30个。在此基础上 ,利用区间作图软件共检测到控制叶片表型性状的QTLs14个 ,位于 9个连锁群上 ;而对于春季萌芽时间共检测到 3个QTLs,分别位于 3个不同的连锁群上。在检测到的 17个QTLs中 ,每一QTL可解释表型变异的 7 6 %～ 15 8%。此外 ,发现控制叶长、叶宽和叶面积等相关性状的QTLs位于相同的基因组区域 ,这些QTLs主要位于毛白杨遗传连锁图的TLG2和TLG11以及毛新杨连锁图的TBLG1连锁群上。据控制叶片表型和春季萌芽时间的QTLs所处的基因组区域 ,可推测叶片表型和春季萌芽时间这两类性状是由各自相应的基因控制。One hundred and twenty progeny of Populus were derived from a cross between female clone “TB01' (Populus tomentosa×P. bolleana) and the male clone “LM50' (P. tomentosa). This population was used to detect quantitative trait loci (QTLs) affecting leaf morphology and spring bud flush. A total of 393 AFLP markers were identified and used to construct a parental specific genetic map using pseudo-test-cross mapping strategy. The total genome length corresponding to 3 265.1 cM for the clone “LM50' map and 1 992 cM for the clone “TB01' map with 4～30 markers per linkage group was obtained. Fourteen QTLs controlling leaf morphology were identified on nine linkage groups, and 3 QTLs affecting spring bud flush were detected on three linkage groups with interval mapping software. The phenotypic variance explained by each QTL ranged from 7.6% (on TBLG14) to 15.8% (on TLG9). Co-localization of QTLs controlling correlated traits such as leafblade length, leafblade width and leafblade area were mainly found on linkage groups TLG2 and TLG11 in the genetic map of clone “LM50', and on linkage group TBLG1 in the genetic map of clone “TB01'. Based on the genomic regions of QTLs for leaf morphology and spring bud flush, these two traits seem to be controlled by separate genes.

关 键 词：毛新杨 毛白杨 QTL 春季 萌芽 叶片 位点 连锁群 基因组 框架图 

分 类 号：S792[农业科学—林木遗传育种] S511[农业科学—林学]