铜绿假单胞菌增强型绿色荧光蛋白标记的研究  

EGFP MARKER OF PSEUDOMONAS AERUGINOSA

作　　者：魏明宝[1] 崔长征[1] 方呈祥[2] 张甲耀[1] 王海[1] 王亚芬[2] 

机构地区：[1]武汉大学资源与环境科学学院,武汉430079 [2]武汉大学生命科学学院,武汉430072

出　　处：《应用与环境生物学报》2005年第1期74-77,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金 (No. 20077021 )资助~~

摘　　要：　根据增强型绿色荧光蛋白(EGFP)基因设计了上下游引物P1和P2,通过对铜绿假单胞菌菌株X3绿色荧光蛋白标记研究,构建了铜绿假单胞菌标记系统,获得带有EGFP标记的基因工程菌X9,为进行相关的跟踪研究创造了条件.该菌株绿色荧光标记性能稳定.通过转化子基因组DNAPCR和斑点杂交检测,外源EGFP基因存在于转化子染色体上. 图 6参Up primer P1 and down primer P2 were designed according to enhancing green fluorescent protein (EGFP) gene. With the study of Pseudomonas aeruginosa markered with EGFP, the system of P. aeruginosa marker was constructed and recombined strain of P. aeruginosa/EGFP was gained. The capability of EGFP marker was stable. The strain was examined by genomic DNA PCR and spot blotting, and the transformed EGFP gene lied in chromosome of P. aeruginosa. Fig 6, Ref 14

关 键 词：铜绿假单胞菌 EGFP PCR 斑点杂交 

分 类 号：Q78[生物学—分子生物学]