溶胶-凝胶法与两步沉淀法制备的镁黄长石粉体的体外生物活性比较  

COMPARISON OF IN VITRO BIOACTIVITY FOR AKERMANITE POWDERS PREPARED BY SOL-GEL AND TWO-STEP PRECIPITATION METHOD

作　　者：吴成铁[1] 常江[1] 汪正[2] 曾毅[2] 钱伟君[2] 

机构地区：[1]中国科学院上海硅酸盐研究所生物材料与组织工程中心 [2]中国科学院上海硅酸盐研究所无机材料分析与测试中心,上海200050

出　　处：《硅酸盐学报》2005年第2期225-229,共5页Journal of The Chinese Ceramic Society

基　　金：上海市科学技术委员会(02JC14009)资助项目。

摘　　要：通过溶胶凝胶法和两步沉淀法分别合成镁黄长石(Ca2MgSi2O7)粉体,并通过模拟体液浸泡对这两种方法合成的镁黄长石的体外生物活性进行比较。用X射线衍射、扫描电镜、原子发射光谱(ICP AES)以及pH计分别对浸泡后形成的羟基磷灰石的物相、形貌以及浸泡后模拟体液的离子浓度变化、pH值进行表征。结果表明:两步沉淀法合成的镁黄长石在浸泡 5 d后就能明显检测到羟基磷灰石生成,而溶胶凝胶法合成的镁黄长石在浸泡7 d后才能检测到羟基磷灰石生成;两步沉淀法合成的镁黄长石诱导的羟基磷灰石呈结晶较好的虫状结构,而溶胶凝胶法合成的镁黄长石诱导的羟基磷灰石呈结晶不完整的圆形颗粒结构;而且,两步沉淀法合成的镁黄长石具有更快的Ca离子释放能力。因此,两步沉淀法合成的镁黄长石相对于溶胶凝胶法合成的镁黄长石具有更好的诱导羟基磷灰石形成能力和生物活性。Pure akermanite (Ca2MgSi2O7) powders were synthesized by sol-gel method and two-step precipitation method, respectively. The in vitro bioactivity of akermanite powders obtained from the two different preparing methods was compared by soaking them into simulated body fluid (SBF). Hydroxyapatite (HA) formation on akermanite powders was characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The changes of Ca and P ions concentrations and pH value of SBF after soaking were evaluated by inductively coupled plasma atomic emission spectroscopy (ICP-AES) and pH meter, respectively. The results show that HA is obviously detected on akermanite prepared by two-step precipitation method after only 5 days of soaking, while it is detected after 7 days of soaking for HA on akermanite prepared by sol-gel method. The HA crystalline on akermanite prepared by two-step precipitation method is in worm-like structure, and the crystalline on akermanite prepared by sol-gel method is in unperfect and spherical structure. The ability of Ca releasing from akermanite prepared by two-step precipitation method is higher than that of akermanite prepared by sol-gel method. Therefore, the in vitro bioactivity of akermanite prepared by two-step precipitation method is better than that prepared by sol-gel method.

关 键 词：镁黄长石 溶胶-凝胶 两步沉淀 生物活性 

分 类 号：R318[医药卫生—生物医学工程] TB332[医药卫生—基础医学]