不同剂量HBV疫苗接种产生的细胞毒T淋巴细胞反应  

Cytotoxic T cell responses to hepatitis B virus (HBV) surface antigen vaccine in murine

作　　者：施理 易学瑞 陈劼[2] 刘惠萍 祖萍 孔祥平 张宜俊 

机构地区：[1]全军肝病中心全军肝病重点实验室,广州510602 [2]广州中医药大学

出　　处：《胃肠病学和肝病学杂志》2005年第1期56-58,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：广东省重点科技项目 (99M0 4 81 1G)

摘　　要：目的　研究不同剂量HBV疫苗接种产生的细胞毒T淋巴细胞 (cytotoxicTlymphocytes ,CTL)反应。。方法　 10 0只BALB/c小鼠随机分为 5组 :0 . 65、1 . 2 5、2 . 5、5组小鼠腹腔分别接种 0 . 65、1 . 2 .5、2 . 5、5 μg的HBV疫苗 ,其中一半小鼠 2周后加强免疫1次 :对照组小鼠同时按种 5 μg佐剂。分别在初次免疫后 4周或加强免疫后 2周 ,分离小鼠脾T淋巴细胞 ;体外用HBV疫苗特异性CTL表位多肽S2 8 3 9 刺激 ;用特异性多肽S2 8 3 9、51 Cr标记的P815细胞作为靶细胞 ;4小时51 Cr释放实验检测CTL反应。结果　开始时随接种剂量增大CTL反应逐步增强 ,至 1 . 2 5 μg达到最大 ,以后又逐步减弱 ;加强免疫显著增强CTL反应。 结论　不同剂量HBV疫苗接种产生的CTL反应强弱不同 ,而且加强免疫能够提高CTL反应。Objective Hepatitis B virus(HBV) specific cytotoxic T lymphocytes (CTL) responses induced by various dose of immunization in murine were assayed.Methods One hundred BALB/c mouse in different groups were vaccinated intraperitoneally with adjuvant(control group) or different dose of HBV vaccine(0.65, 1.25, 2.5, 5 μg experimental group, respectively). Half of the mouse in each group received boost inoculation. Four weeks after primary injection or 2 weeks after boost inoculation, spleen cells were stimulated in vitro with specific peptides, which represents a Ld-restricted CTL epitope of the HBV vaccine in H-2d BALB/c murine. The specific cytotoxic reactivity was tested against P815 cells impulsed with specific peptides and labeled with 51Cr. Four hours release assay of 51Cr was performed.Results The CTL response was different in various groups. It was greatest in group inoculated with 1.25μg HBV vaccine.Conclusions The strength of CTL activity is dependent on the inoculating dose and times of the injection.

关 键 词：HBV疫苗 加强免疫 不同剂量 CTL反应 细胞毒T淋巴细胞 BALB/C小鼠 接种 多肽 靶细胞 特异性 

分 类 号：R512[医药卫生—内科学] R373[医药卫生—临床医学]