HBcAg刺激对HBV慢性携带者外周血单个核细胞分泌IFN-γ的影响  

The effect of HBcAg and HBsAg to the production of IFN-γ of pBMCs from the HBV chronic carrier

作　　者：梁晓岳 施理 易学瑞 陈劼[3] 刘惠萍 祖萍 孔祥平 张宜俊 

机构地区：[1]宁波传染病医院,宁波315010 [2]全军肝病中心全军肝病重点实验室 [3]广州中医药大学

出　　处：《胃肠病学和肝病学杂志》2005年第1期87-89,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：广东省重点科技项目 (99M0 4 81 1G)

摘　　要：目的　研究乙型肝炎病毒 (hepatitisBvirus ,HBV)慢性携带者外周血单个核细胞 (peripheralbloodmononuclearcells ,PBM Cs)受HBV核心抗原 (HBVcoreantigen ,HBcAg)、表面抗原 (HBVsurfaceantigen ,HBsAg)刺激后分泌γ-干扰素 (interferonγ ,IFN -γ)的免疫反应。方法　临床上选择 3 2例慢性HBV携带者 ,其中转氨酶升高的HBV免疫清除期患者及转氨酶正常的HBV免疫耐受期患者各半 ,常规密度梯度离心方法分离PBMCs ,体外用HBcAg、HBsAg刺激 ,并以 164 0培养基作为对照 ,用酶联免疫斑点 (enzymelinkim munalSpot,ELISPOT)方法检测PBMCs分泌IFN 0γ的免疫反应。结果　与 164 0刺激的对照相比 ,87 .5 %(14 /16例 )免疫消除期的HBV携带者PBMCs受HBcAg刺激 ,IFN -γ分泌实验阳性 ,而所有免疫耐受期的HBV携带者PBMCs受HBcAg刺激 ,IFN -γ分泌实验阴性 ,二者之间有显著差异 (P <0. 0 5 )。结论　HBV免疫清除期与免疫耐受期患者PBMCs对HBcAg刺激的免疫反应不同。Objective To investigate interferon_γ production of peripheral blood mononucler cells in response to hepatitis B core antigen and surface antigen in chronic hepatitis B infection.Methods Thirty-two patients chronically infected with HBV, 16 patients in clerance period(with the increased alanine aminotransferase) and 16 patients in tolerance period(with the normal alanine aminotransferase), were included. The peripheral blood mononuclear cells (PBMCs) from the patients separated by density gradient centrifugation were stimulated with HBV core antigen (HBcAg) and HBV surface antigen (HBsAg) separately in vitro. The production of interferon_γ (IFN-γ) was measured by method of enzyme link immunal spot (ELISPOT). Results Compared with cells stimulated with 1640, the production of IFN-γ rised significantly in cells stimulated with HBcAg(87.5%,14/16) in patients in clerance period .Conclusions The response of the production of IFN-γ stimulated with HBcAg for the patients in clerance period is significant different from those in tolerance period.

关 键 词：HBV携带者 PBMC IFN-Γ 刺激 分泌 慢性携带者 免疫耐受期 密度梯度离心 斑点 培养基 

分 类 号：R512.62[医药卫生—内科学] R392[医药卫生—临床医学]