加味玉屏风散对创伤应激小鼠CD4^+CD25^+调节性T细胞影响的实验研究  

Effect of modified Yu Ping Feng Pulvis on the CD4^+CD25^+ reglatory T cell in traumatic stress mice

作　　者：曾广仙 刘俊英 熊金蓉 代丽红 赵璐 杨燕萍[2] 陈新 沈关心[4] 

机构地区：[1]解放军第457医院,武汉30012 [2]上海复旦大学遗传学系 [3]美国国家卫生研究院 [4]华中科技大学同济医学院免疫学系

出　　处：《中华微生物学和免疫学杂志》2005年第1期69-72,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　观察创伤应激状态下及口服加味玉屏风散 (玉屏风散加党参 )后对小鼠CD4 +CD2 5 + Tr细胞影响。方法　采用小鼠截肢应激模型 ,将 5 0只BALB c小鼠随机分为 5组 :正常对照组(A)、应激对照组 (B)、应激 +加味玉屏风散高 (C)、中 (D)、低 (E)剂量组。采用流式细胞仪检测小鼠外周血和脾细胞悬液中CD4 + CD2 5 - T、CD4 - CD2 5 + T、CD4 + CD2 5 + Tr的细胞数。结果　创伤后 72h小鼠外周血及脾细胞悬液中CD4 + CD2 5 + Tr细胞比例明显升高 (P <0 .0 1 ) ,CD4 + CD2 5 - T细胞、CD4 - CD2 5 + T细胞比例明显降低 (P <0 .0 1 ) ;给予加味玉屏风散后各组小鼠外周血及脾细胞悬液中CD4 + CD2 5 + Tr细胞比例明显降低 (P <0 .0 1 ) ,CD4 + CD2 5 - T细胞、CD4 - CD2 5 + T细胞比例明显升高 (P <0 .0 1 ) ;其中C组与A组比较差异无统计学意义 (P >0 .0 5 )。结论　创伤后小鼠CD4 + CD2 5 + Tr表达增强 ,加味玉屏风散可有效抑制其表达。Objective To explore the effects of modified Yu Ping Feng Pulvis(YPFP) on CD4+CD25+ regulatory T cells in the traumatic stress mice. Methods With the mice amputee model, 50 BALB/c mice were divided into 5 groups randomly. Group A(normal control group), group B(traumatic stress group), group C(traumatic stress + high dose YPFP group), group D(traumatic stress + medial dose YPFP group) and group E(traumatic stress + low dose YPFP group). Then the counts of T cell with CD4+CD25-, CD4-CD25+ and CD4+CD25+ in peripheral-blood and splenic cell suspension were sorted out in each group respectively by FCM. Results After 72 h of trauma, the proportion of CD4+CD25+ regulatory T cell was significantly increased (P<0.01), and the proportions of T cell with CD4-CD25+ and CD4+CD25+ were remarkably reduced (P<0.01) in each group; But after taken the modified YPFP, there was no statistical difference between the results of group C and group A (P>0.05). Conclusion The expression of CD4+CD25+ in regulatory T cell was elevated after trauma, but modified YPFP may inhibit this expression. [

关 键 词：CD25^+ CD4^+ BALB/c小鼠 加味玉屏风散 T细胞 悬液 脾细胞 创伤应激 表达 截肢 

分 类 号：R285[医药卫生—中药学]