异莲心碱对苯肾上腺素诱导猪冠脉平滑肌细胞增殖的影响(英文)  

Effects of isoliensinine on proliferation of porcine coronary arterial smooth muscle cells induced by phenylephrine

作　　者：肖军花[1] 张延琳[2] 丁丽丽[1] 冯秀玲[1] 王嘉陵[1] 

机构地区：[1]华中科技大学同济医学院药理学系 [2]华中科技大学同济医院口腔学系,湖北武汉430030

出　　处：《药学学报》2005年第2期105-110,共6页Acta Pharmaceutica Sinica

基　　金：ScientificFoundationofMinistryofHealth(98DJ15 87).

摘　　要：目的　探讨异莲心碱(IL)对苯肾上腺素(Phen)诱导猪冠脉血管平滑肌细胞 (CASMCs)增殖作用的影响及其机制。方法　改良MTT比色法、免疫组织细胞化学技术和WesternBlotting等方法。结果　Phen( 0 .1μmol·L-1 )明显诱导CASMCs增殖,生长因子、原癌基因与hsp70蛋白表达增多。IL(0 03-3μmol·L-1 )浓度依赖性地抑制Phen促猪CASMCs增殖作用。IL(0 .1μmol·L-1 )可抑制Phen诱导的PDGF β和bFGF的蛋白表达增多,并可抑制c fos, c myc和hsp70蛋白表达增多。结论　IL具有抗Phen诱导猪CASMCs增殖的作用,其抗增殖机制与抑制生长因子PDGF β,bFGF及原癌基因c fos,c myc和hsp70的增强表达有关。Aim To investigate the inhibitory effects and mechanism of action of isoliensinine (IL) on the proliferation of porcine coronary arterial smooth muscle cells (CASMCs) induced by phenylephrine (Phen) and its mechanisms of action. Methods MTT assay, immunohistochemical method and Western blotting were adopted. Results IL (0.03-3 μmol·L-1) could inhibit the CASMCs proliferation induced by Phen (0.1 μmol·L-1) in a concentration-dependent manner. IL (0.1 μmol·L-1) antagonized Phen-induced overexpression of PDGF-β and bFGF from 0.545±0.026 and 0.47±0.03 to 0.458±0.019 and 0.376±0.017 (P<0.01, P<0.01). IL (0.1 μmol·L-1) also decreased c-fos, c-myc and hsp70 overexpression induced by Phen from 0.57±0.04, 0.44±0.04 and (173±36)% to 0.46±0.05, 0.372±0.021 and (115±35)% respectively (P<0.01, P<0.01, P<0.01). Conclusion IL exerted antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms were related to decrease the overexpression of growth factors (PDGF-β, bFGF), protooncogene (c-fos, c-myc) and hsp70.

关 键 词：异莲心碱 苯肾上腺素 细胞增殖 原癌基因 热休克蛋白70 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]