气相色谱/燃烧炉/同位素比值质谱方法对尿中去氢表雄酮及其代谢物的检测  

Detection of the metabolites of dehydroepiandrosterone in urine with gas chromatography-combustion-isotope ratio mass spectrometry

作　　者：王静竹[1] 吴侔天[1] 张亦农[1] 刘欣[1] 杨志勇[1] 

机构地区：[1]国家体育总局运动医学研究所,北京100029

出　　处：《药学学报》2005年第2期159-163,共5页Acta Pharmaceutica Sinica

基　　金：科技部奥运科技专项课题资助项目(2002BA04B01).

摘　　要：目的　测定服用去氢表雄酮制剂后人尿中去氢表雄酮及其代谢物的同位素比值 (13C与12C含量比值的相对值,以δ表示)进行测定,以检测尿中类固醇的来源。方法　采用固相萃取、酶解、薄层色谱等方法对尿样中内源性类固醇激素进行提取、酶解游离型、分离,再经气相色谱 /燃烧炉 /同位素比值质谱方法测定去氢表雄酮、其代谢物内源性类固醇激素、内源性类固醇参照物的同位素比值的相对值 (δ值)。将被检测物与参照物δ值之比,与判别标准比较进行来源判定。结果　服用去氢表雄酮制剂后人尿中内源性类固醇激素代谢物δ值比未使用去氢表雄酮制剂正常值降低,并提示有外源性类固醇摄入。结论　本法可以检测尿中去氢表雄酮或其代谢物的来源。Aim To establish a method to determine the isotope ratios of 13C to 12C of dehydroepiandrosterone and its metabolites in urine, for detecting the source of dehydroepiandrosterone or its metabolites. Methods Preliminary separation of endogenous anabolic androgenic steroids could be achieved using solid phase extraction, enzymolysis and thin layer chromatography. The source of dehydroepiandrosterone and other endogenous anabolic androgenic steroids could be detected by their δ values with gas chromatography-combustion-isotope ratio mass spectrometry. Results The δ values of some metabolites of dehydroepiandrosterone reduced after the administration of dehydroepiandrosterone preparation. In these cases the data indicated that exogenous anabolic androgenic steroids were administrated. Conclusion The source of dehydroepiandrosterone or its metabolites in urine could be detected by measuring their δ values with this method.

关 键 词：气相色谱/燃烧炉/同位素比值质谱法 去氢表雄酮 尿 

分 类 号：R917[医药卫生—药物分析学]