拟除虫菊酯类农药多残留酶免疫分析方法的建立  

Development of Enzyme Immunoassays for Pyrethriods

作　　者：骆爱兰[1] 余向阳[1] 张存政[1] 祝树德[2] 刘贤进[1] 

机构地区：[1]江苏省农业科学院食品质量安全与检测研究中心,南京210014 [2]扬州大学植物保护系,扬州225009

出　　处：《中国农业科学》2005年第2期308-312,共5页Scientia Agricultura Sinica

基　　金：国家"863"计划资助项目(2001A246031);江苏省重点攻关资助项目(BE2003305)

摘　　要：以间苯氧基苯甲酸(PBA)为半抗原,与牛血清蛋白偶联后免疫新西兰大白兔,获得对多种菊酯类农药有特异性的广谱性抗体。以6-氨基己酸法合成PBA与OVA之间含有六碳骨架的桥的偶联物作包被原,建立并优化了间接竞争ELISA检测方法,对PBA最低检出限达0.193μg·ml-1,抑制中浓度为3.707μg·ml-1,且对氯菊酯、甲氰菊酯、氯氰菊酯、三氟氯氰菊酯、溴氰菊酯均有特异性识别,对氰戊菊酯识别弱,抑制中浓度分别为4.066、4.232、4.286、4.874、8.979、77.360μg·ml-1。结果表明,该抗体可用于多种菊酯类农药残留筛选检测。phenoxybenzoic acid (PBA) was used as generic hapten in order to generate broad specificity antibody against a group of pyrethriod insecticides. Assay performance was improved by use of a coating antigen, which possessed a six-carbon spacer arm between the hapten and carrier protein. Typical detection limits for PBA was 0.193 μg·ml-1with an I50 value of 3.707 μg·ml-1. Different cross-reactions with the closely related pyrethriods,including permethrin, fenpropathrin, cypermetnrin, cyhalothrin and deltamethrin, were observed, with I50 value of 4.066, 4.232,4.286,4.874,8.979 μg·ml-1, respectively; There was a low cross reactivity for fenvalerate with an I50 value of 77.360 μg·ml . The result developed in this study indicated the antibody was -1 practicable to screen the residues of pyrethriod insecticides.

关 键 词：酶免疫分析 抗体 6-氨基己酸 大白兔 抑制 氯菊酯 偶联物 残留 药残 菊酯类农药 

分 类 号：S481[农业科学—农药学]