IBDV超强毒株感染SPF鸡后法氏囊淋巴细胞凋亡及Bax和Bcl-2蛋白动态表达的研究  

The Apoptosis and Dynamic Expression of Bax and Bcl-2 in Lymphocytes of Bursa of SPF Chickens Infected with SNJ93 Strain

作　　者：刘玉锋[1] 佘锐萍[1] 李慧姣[2] 靳红[1] 梁明珍 刘玉如[1] 寸钢彪[1] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]中国兽医药品监察所,北京100081 [3]北京兽医实验诊断所,北京100101

出　　处：《中国农业科学》2005年第2期405-409,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30270995;30471301);北京市自然科学基金资助项目(6022012)

摘　　要：为证实传染性法氏囊炎超强毒SNJ93株感染SPF鸡后1～14　d内法氏囊淋巴细胞中Bcl-2、Bax蛋白的动态表达与淋巴细胞凋亡的关系。应用透射电镜观察、TUNEL法标记检测凋亡的淋巴细胞,用免疫组织化学方法检测Bcl-2、Bax蛋白。结果表明,感染后1～7　d可见到大量凋亡的淋巴细胞,其中感染后3～5　d最多。感染后1～9d　Bcl-2蛋白显著高于同时点的对照组(P<0.01);感染后1～5　d　Bax蛋白明显高于同时点的对照组,差异极显著(P<0.01);感染后第7天高于同时点的对照组,差异显著(P<0.05);　Bcl-2/Bax比率在感染后第1天低于同时点的对照组(P<0.05);3～5　d明显低于同时点的对照组(P<0.01)。SNJ93感染SPF雏鸡后1～7　d内法氏囊淋巴细胞会发生大量凋亡,Bcl-2/Bax能准确反映淋巴细胞的凋亡程度。Apoptosis and dynamic expression of their regulatory genes Bcl-2 and Bax in lymphocytes of bursa of 21-day –old SPF chickens infected with SNJ93 strain from the first to fourteenth day post infection were investigated. Apoptosis was observed by electron microscope and apoptosis rate was assessed by TUNEL method. The expression of Bcl-2, Bax was investigated by immunohistochemical ABC method. The apoptosis of lymphocytes in bursa of chickens infected with SNJ93 strain from the first to seventh day post infection significantly increased compared with that of normal SPF chickens (P<0.01).The expression of BCL-2 significantly increased in lymphocytes of bursa of SPF chickens infected with SNJ93 strain from the first to nineth day post infection compared with those of normal chickens (P<0.01). The expression of Bax significantly increased from the first to seventh day post infection (P<0.01 or P<0.05).The ratio of bcl-2 and Bax significantly decreased from the first to fifth day post infection (P<0.05 or P<0.01). A great deal of disappearance of lymphocytes of bursa of SPF chickens infected with SNJ93 strain from the first to seventh day was related to apoptosis .The ratio of Bcl-2 and Bax could precisely reflect the degree of apoptosis of lymphocytes of bursa.

关 键 词：感染后 对照组 淋巴细胞凋亡 凋亡 动态表达 BAX蛋白 Bcl-2蛋白 法氏囊 SPF鸡 超强毒株 

分 类 号：S852.3[农业科学—基础兽医学]