李矮缩病毒RT-PCR方法建立及检测应用  

Establishment and Application of Detection of Prune Dwarf Virus (PDV) by RT-PCR

作　　者：侯义龙[1] 杨俊玲[2] 李春敏[3] 

机构地区：[1]大连大学生物工程学院,大连116622 [2]中国农业科学院果树研究所,兴城125100 [3]河北省农林科学院昌黎果树研究所,昌黎066600

出　　处：《中国农业科学》2005年第2期425-427,共3页Scientia Agricultura Sinica

基　　金：辽宁省博士启动基金资助项目(20021052);辽宁省教育厅高等学校科研项目(2023901007)

摘　　要：以感病和健康指示植物GF305的总RNA为模板,进行cDNA的合成和PCR扩增,结果从感病材料中扩增出与预期的172bp大小一致的目的片段,而健康的材料无此扩增产物。对此PCR扩增产物克隆测序,进一步佐证了RT-PCR检测结果。经过多次试验验证该检测体系,都可得到很好的重演性,从而建立了李矮缩病毒快速、灵敏、准确的RT-PCR检测技术,并进行了实际应用。A pair of primers was synthesized based on the nucleotide sequence of the coat protein gene of prune dwarf virus (PDV).The expected size 172bp was amplified by RT-PCR from the infected samples, while no amplified products from the healthy samples.The amplified products were cloned and sequenced. The result showed that the detection system was stable.Thus a rapid, sensitive and accurate method for PDV identification and detection by RT-PCR has been built up and were used to detect whether some fruit trees in orchards were infected by PDV or not.

关 键 词：矮缩病 RT-PCR检测技术 指示植物 扩增产物 PCR扩增 病毒 RT-PCR方法 健康 GF 灵敏 

分 类 号：S436.62[农业科学—农业昆虫与害虫防治]