天智颗粒对叠氮钠诱导脑损伤大鼠纹状体细胞外乙酰胆碱和儿茶酚胺含量的影响  

Effects of Tianzhi Keli on extracellular acetylcholine and catecholamine levels in striatum of rats with neuromitochondrial impairment

作　　者：孙晓芳[1] 王巍[1] 王丹巧[1] 赵德忠[1] 杜贵友[2] 

机构地区：[1]中国中医研究院西苑医院老年病研究所,北京100091 [2]中国中医研究院中药研究所,北京100700

出　　处：《中国中药杂志》2005年第2期141-145,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目 (3 0 171185 ) ;北京自然科学基金资助项目 (70 12 0 3 3 )

摘　　要：目的 :观察天智颗粒 (TZ)对脑线粒体损伤大鼠纹状体细胞外乙酰胆碱 (ACh)和儿茶酚胺类递质的影响 ,探讨促智中药TZ的神经保护作用机理。方法 :应用微透析技术和高效液相色谱相结合 ,建立清醒自由活动大鼠脑内灌流叠氮钠 (NaN3 )造成局部脑区线粒体能量代谢障碍的急性模型 ,动态监测TZ对叠氮钠灌流大鼠脑细胞外ACh、胆碱 (Ch)和儿茶酚胺类递质水平的影响。结果 :NaN3 灌流后 ,大鼠纹状体细胞外ACh、去甲肾上腺素(NE)、肾上腺素 (E)、多巴胺 (DA)、3,4 二羟基苯乙酸 (DOPAC)和高香草酸 (HVA)水平均显著下降 (P <0.0 5～ 0. 0 1) ,Ch水平则明显升高 (P <0.0 1) ;停止NaN3 灌流后 ,各递质水平逐渐恢复。TZ可延缓叠氮钠灌流大鼠脑内ACh水平的降低 ;促进Ch水平的恢复 ;与都可喜合用升高叠氮钠灌流大鼠ACh水平的作用显著优于单用TZ或都可喜。TZ还有延缓儿茶酚胺类递质水平降低和促进其恢复的趋势 ,与都可喜合用可促进DOPAC水平的恢复 ,对DA的代谢异常具有积极的调节作用。结论 :TZ对线粒体损伤 ,能量代谢障碍引起的胆碱能神经系统的损伤具有保护作用 ,并与都可喜有协同作用 ;对肾上腺素能、多巴胺能神经系统的损伤 。Objective: To investigate the effects of Tianzhi Keli(TZ) on acetylcholine(ACh) and catecholamine levels in striatum of rats with neuromitochondrial impairment, and try to find out the neuroprotective mechanism of TZ. Method: The microdialysis and high performance liquid chromatography(HPLC)-post column Immobilized enzyme reactor(IMER)-electrochemical detection(ED) were used to establish a model of mitochondrial energy metabolism impairment which induced by perfusion with sodium azide(NaN_3), and measure continuously the effects of TZ on extracellular ACh, choline(Ch) and catecholamine of model rats. Result: After perfusion with NaN_3, ACh, noradrenalin(NE), adrenaline(E), dopamine(DA), 3, 4-Dihydroxyphenyl-aletic(DOPAC), and homovanillic acid(HVA) levels were decreased obviously(P<0.05～0.01), while Ch level was increased distinctly(P<0.01). Transmitters levels were recovered individually after stop the perfusion with NaN_3.TZ can postpone the decrease of ACh and advance the recover of Ch. The effect of TZ coupled with duxil on increasing ACh level is more obviously than effect of TZ or duxil. TZ is also showing a tendency to postpone the decrease of catecholamine and advance its recovery. TZ coupled with duxil can advance the recovery of DOPAC and adjust the metabolic abnormity positively. Conclusion: TZ has effect on protecting impairment of choline neurosystem, which induced by damage of mitochondrion and abnormity of energy metabolism; coupled with duxil have synergistic action. TZ also has tendency to protect the impairment of epinephrine and dopamine neurosystem.

关 键 词：大鼠 灌流 叠氮钠 纹状体 递质 儿茶酚胺类 都可喜 体细胞 胆碱 含量 

分 类 号：R285.5[医药卫生—中药学]