小檗碱对3T3-L1脂肪细胞脂联素表达的影响  

Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte

作　　者：谷卫[1] 曾文衡[1] 胡海英[1] 

机构地区：[1]浙江大学医学院附属第二医院,浙江杭州310009

出　　处：《中国中药杂志》2005年第4期286-288,共3页China Journal of Chinese Materia Medica

摘　　要：　目的:探讨小檗碱和胰岛素对3T3-L1脂肪细胞脂联素表达的影响。方法:检测分别经小檗碱及胰岛素处理后3T3 L1脂肪细胞脂联素表达水平改变,以β actin为内对照,半定量法RT PCR测定脂联素mRNA表达。结果:经小檗碱(10μmol·L-1)干预后3T3 L1脂肪细胞脂联素表达显著增加(P<0.05),加入胰岛素后脂联素的表达受到抑制,高浓度胰岛素有显著抑制作用(P<0.05)。结论:体外实验中,小檗碱使3T3-L1脂肪细胞脂联素的表达增加,而胰岛素可抑制小檗碱增加脂联素表达的作用。Objective: To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte. Method:The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours,the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using β-actin as internal reference. Result: The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only(P<0.05),the effect of berberine was inhibited by high concentration insulin(P<0.05). Conclusion: in vitro,berberine increases the expression of adiponectin in 3T3-L1 adipocyte,insulin inhibits the effect of berberine.

关 键 词：脂联素 小檗碱 3T3-L1脂肪细胞 表达 胰岛素 体外实验 影响 增加 目的 作用 

分 类 号：R285.5[医药卫生—中药学]