淫羊藿苷对人成骨细胞增殖与分化的影响  被引量:111

Effects of epimedium pubescens icariine on proliferation and differentiation of human osteoblasts

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作  者:殷晓雪[1] 陈仲强[1] 党耕町[1] 马庆军[1] 刘忠军[1] 

机构地区:[1]北京大学第三医院,北京100083

出  处:《中国中药杂志》2005年第4期289-291,共3页China Journal of Chinese Materia Medica

摘  要: 目的:观察淫羊藿苷对人成骨细胞增殖和分化的影响。方法:将人骨髓基质干细胞定向诱导分化为成骨细胞,取第3,4代细胞,MTT法观察淫羊藿苷对人成骨细胞的增殖作用,碱性磷酸酶(ALP)比活性测定观察淫羊藿苷对人成骨细胞分化的影响,RT-PCR方法检测淫羊藿苷对人成骨细胞 BMP-2 mRNA表达的影响。结果:浓度为20μg·mL-1的淫羊藿苷能够显著促进人成骨细胞的增殖和分化,且使BMP-2 mRNA的表达升高。结论:淫羊藿苷具有促进人成骨细胞增殖和分化的作用,这一作用可能与其升高人成骨细胞BMP 2 mRNA的表达有关。Objective: To study the effects of epimedium pubescens icariine on the proliferation and differentiation of human osteoblasts. Method: Human osteoblasts were obtained by inducting human marrow mesenchymal stem cells (hMSCs) directionally. MTT was used to observe the proliferation and activity of ALP was assayed to observe the differentiation of the third passage human osteoblasts cultured in vitro. The expression of BMP-2 mRNA was checked by RT-PCR. Result: Epimedium pubescens icariine at the dose of 20 μg·mL^(-1)increased greatly the proliferation and differentiation of human osteoblasts and promoted the expression of BMP-2 mRNA. Conclusion: Epimedium pubescens icariine enhances isgnificantly the proliferation and differentiation of human osteoblasts, which may be mediated by increasing the expression of BMP-2 mRNA.

关 键 词:人成骨细胞 淫羊藿苷 增殖 BMP-2 分化 观察 升高 浓度 RT-PCR方法 RNA 

分 类 号:R285.5[医药卫生—中药学]

 

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