核因子-κB在四逆汤预处理诱导大鼠心肌延迟预适应中的作用  

Role of NF-κB in the induction of delayed preconditioning in rat heart by pretreatment with Sini decoction

作　　者：刘颖[1] 吴伟康[1] 陈晨[2] 刘克玄[1] 

机构地区：[1]中山大学中西医结合研究所 [2]中国人民解放军第421医院检验科,广东广州510318

出　　处：《中国中药杂志》2005年第6期453-456,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(39770936)

摘　　要：目的:探讨四逆汤能否诱导心肌延迟预适应及其可能机制。方法:SD大鼠分为假手术组、缺血再灌注组、延迟缺血预处理组、四逆汤预处理组。延迟缺血预处理组采用经典大鼠冠脉结扎,缺血5min,再灌5min,反复循环3次,24h后缺血1h,再灌1h。四逆汤预处理组给予四逆汤灌胃(5g·kg-1·d-1)连续3d,末次灌药24h后缺血1h,再灌1h。以心肌梗死面积为评价指标并测定心肌中超氧化物歧化酶的活性、丙二醛的含量,通过RT PCR检测MnSODmRNA及Cu ZnSODmRNA的表达并利用免疫组织化学染色法检测大鼠心肌NFκB亚单位P65的表达。结果:延迟缺血预处理组及四逆汤预处理组心肌梗死面积明显小于缺血再灌注组,MDA的含量低于缺血再灌注组,SOD活性和MnSODmRNA的表达高于缺血再灌注组,NFκB发生核转位且蛋白表达显著高于缺血再灌注组。缺血再灌注组、延迟缺血预处理组及四逆汤预处理组的Cu ZnSODmRNA表达均下降,3组之间无差异。结论:四逆汤能诱导心肌延迟预适应,其机制可能与NFκB的激活相关。Objective: The present study was designed to investigate whether Sini decoction (SND) containing several chinese medicinal herbs, could induce delayed preconditioning-like effect in rat heart and the possible mechanism. Method: In anesthetized open-chest Sprague Dawley rats, left anterior descending coronary artery (LAD) was occluded for 1 h and reperfused for 1 h. All rats were randomly divided into four groups: Sham (n=8) group in which the surgical procedure was identical to other groups,but the LAD ligature was not ligated; I/R group (n=8) in which the LAD of rat hearts was subjected to the index occlusion; IPC/SWOP group(n=8) in which the LAD was occluded for three cycles of 5-minute episodes of preconditioning ischemia followed by 5-minute episodes of reperfusion 24 hours prior to the index occlusion; SND group (n=8) in which rats were pretreated with Sini decoction (5 g·kg^(-1) d^(-1)) for three days,the last treatment was pretreated 24 h before the index occlusion. Myocardial infarct size, the activity of superoxide dismutase(SOD) and the content of malondialdehyde (MDA) in myocardial tissue were measured. The mRNA expression of MnSOD and Cu-ZnSOD were detected by RT-PCR. The expression of P65 subunit of NF-κB was determined by immunohistochemistry assay. Result: As compared with I/R group, myocardial infarct size and the amount of MDA in myocardial tissue were significantly decreased, the activity of SOD and the mRNA expression of MnSOD were increased, the translocation of NF-κB was detected and furthermore the expression of NF-κB was significantly elevated in SND group as well as in IPC/SWOP group. The mRNA expression of Cu-ZnSOD was lowered in I/R, SND and IPC/SWOP groups, and there was no significant difference among these groups. Conclusion: The results suggest that Sini decoction can induce delayed preconditioning-like effect in rat heart and the activation of NF-κB may play an important role in it.

关 键 词：四逆汤 缺血再灌注 心肌 延迟预适应 缺血预处理 大鼠 诱导 RT-PCR检测 B亚单位 SOD 

分 类 号：R285[医药卫生—中药学]