不同危险分组ALL细胞PI3K/AKT磷酸化激活的初步研究  

Preliminary Study on Constitutive Activation of Phosphatidyl- Inositide 3 Kinase/AKT (PI3K/AKT) in Patients with Acute Lymphoblastic Leukemia of Different Risk Classifications

作　　者：梁爱斌 [1] 李莉 [1] 谢晓恬 [1] 江华 [2] 叶辉 [2] 赵金彩 [2] GU Long-jun  

机构地区：[1]同济大学附属同济医院儿科,上海,200065 [2]上海第二医科大学附属新华医院儿童医学中心,上海,200192

出　　处：《同济大学学报（医学版）》2005年第1期52-55,共4页Journal of Tongji University(Medical Science)

基　　金：国家自然科学青年基金资助项目 (3 0 10 0 2 0 3 )

摘　　要：目的　观察磷脂酰肌醇 - 3激酶 /蛋白激酶通路 (PI3K/AKT)在不同危险分组的白血病细胞中磷酸化激活的程度 ,初步了解急性白血病细胞PI3K/AKT激活与急淋白血病危险分组的关系。方法　使用蛋白印记分析法(WesternBlot)检测正常健康儿童、初发白血病患儿、缓解后患儿以及难治性白血病患儿白血病骨髓细胞中PI3K/AKT磷酸化激活程度。结果　正常健康儿童骨髓单个核细胞中无PI3K/AKT磷酸化激活 ,10例初发患儿中 3例、10例缓解后患儿 1例有PI3K/AKT磷酸化激活 ,6例难治患儿中 4例有PI3K磷酸化激活。结论　PI - 3K/AKT信号传导通路激活与否在淋巴细胞白血病危险分组和疾病转归的生物学过程中可能起着较重要作用 ,需进一步深入研究。Objective To investigate the constitutive activation of phosphatidyl-inositide 3 kinase/AKT (PI3K/AKT) in the patients with acute lymphoblastic leukemia (ALL) of different risk classifications.Methods Total-AKT (T-AKT) and phosphorylated AKT (P-AKT) were detected by means of Western blotting in healthy donors,patients with de novo acute lymphoblastic leukemia,patients under complete remission and patients with refractory disease.Results T-AKT was detected in all samples studied.No P-AKT was found in healthy donors.P-AKT was detected in only 1 among 10 cases of patients under remission,and in 3 among 10 cases of patients with de novo ALL.In patients of refractory leukemia,4 of 6 cases were found with constitutive activation of P-AKT.Conclusion Phosphatidyl-inositide 3 kinase/AKT (PI3K/AKT) signal transduction pathway may play an important role in the risk classification and the prognosis of ALL after treatment.

关 键 词：急淋白血病 危险分组 磷脂酰肌醇-3激酶 激活 

分 类 号：R72[医药卫生—儿科]