喜树碱抗血管生成作用的研究  

Potential Antiangiogenic Effect of Camptothecin in vitro

作　　者：刘晓明[1] 安利佳[2] 王红梅[1] 包永明[2] 段延龙[2] 林熙然[1] 

机构地区：[1]大连医科大学附属第一医院皮肤科,116011 [2]大连理工大学生物工程系

出　　处：《中华皮肤科杂志》2005年第3期168-169,共2页Chinese Journal of Dermatology

基　　金：辽宁省自然科学基金资助项目(2040736)

摘　　要：目的探讨喜树碱在体外对人脐静脉血管内皮细胞ECV304增殖抑制和凋亡诱导作用。方法将不同浓度的喜树碱作用于ECV304细胞株,用MTT法、光镜、电镜、DNA梯状带检测药物对细胞的增殖抑制和凋亡诱导作用。结果喜树碱能抑制ECV304细胞的增殖。24h,36h,48h最大抑制率分别为67.9%,96.7%,97.2%。形态学观察细胞生长受抑制,可见凋亡小体。凝胶电泳显示典型的DNA梯度带。结论喜树碱体外能抑制人脐静脉血管内皮细胞增殖并诱导其凋亡,证明喜树碱可能具有抗血管生成作用。Objective To investigate the effect of camptothecin (CPT) on the inhibition of proliferation and induction of apoptosis of human umbilical vein endothelial cell line, ECV304, in vitro. Methods ECV304s were incubated with different concentrations of CPT. The inhibitory effect on cellular proliferation was measured by MTT assay. Cellular apoptosis was evaluated by light microscopy, electron microscopy, flow cytometry, and agarose gel electrophoresis. Results CPT could inhibit the proliferation of ECV304. The maximal inhibition rates were 67.9%, 96.7%, and 97.2% at 24 h, 48 h and 72 h, respectively. Having been treated with CPT, ECV304 cells were found to have typical morphologic features of apoptosis. The results of agarose gel electrophoresis showed characteristic DNA ladder after ECV304 were treated with CPT. Conclusions CPT shows inhibitory effect on the proliferation of ECV304 and can induce apoptosis of ECV304. CPT may have a potential antiangiogenic effect.

关 键 词：喜树碱 抗血管生成作用 增殖抑制 人脐静脉血管内皮细胞 ECV304细胞 体外 凋亡诱导 DNA 凝胶电泳 凋亡小体 

分 类 号：R285[医药卫生—中药学]