南通地区慢性丙型肝炎患者的HCV基因分型  

The genotyping of Hepatitis C Virus in Nantong

作　　者：曹金德[1] 王跃国[1] 施英娟[1] 张冬雷[1] 张芹[1] 陈莲英[1] 毛丽萍[2] 王惠民[1] 

机构地区：[1]南通大学附属医院检验医学中心,江苏226001 [2]南通市第三人民医院

出　　处：《中国交通医学杂志》2005年第1期75-76,共2页Chinese Medical JOurnal of Communications

摘　　要：目的 :了解南通地区慢性丙型肝炎患者的HCV基因型分布情况。方法 :将 5 3例抗HCV阳性的血清标本用RT -PCR法检测HCV -RNA ,然后采用膜显色结果判读的基因芯片对 3 2例HCV -RNA阳性者进行基因分型研究。结果 :南通地区HCV感染检出 1b、1a、2a、1b + 1a、1b + 1a + 2a共 5种单一基因型和混合基因型 ,其中以 1b型为主 ,占 5 3 .1%。结论 :南通地区 1b型为优势株 ,2a和 1b + 1a型居次 ;1b + 1a型高于全国其他地区。Objective:To investigate the prevalent condition of Hepatitis C Virus genotyping in Nantong. Methods:53 anti-HCV (+) serum cases were detected by reverse transcription -polymerase chain reaction (RT- PCR), then 32 HCV-RNA (+) cases were determined for their genotypes by HCV genotyping chip.Results:HCV 1b,1a,2a,1b+1a,1b+1a+2a genotypes were found from 53 patients with chronic hepatitis C in Nantong and 1b was the main one (53.1%).Conclusions:It was suggested that HCV 1b is the main prevalent type in Nantong, followed by HCV 2a and 1b+1a type. The percentage of HCV 1b+1a type was higher than other areas.

关 键 词：丙型肝炎 病毒基因 慢性肝炎 

分 类 号：R446.11[医药卫生—诊断学]