鳞球茎茎线虫实时荧光PCR检测技术的研究  

Detection of Ditylenchus dipsaci by real time PCR.

作　　者：王翀  葛建军  陈长发  

机构地区：[1]中国检科院动植物检疫研究所,北京,100029 [2]青岛出入境检验检疫局

出　　处：《植物检疫》2005年第1期11-14,共4页Plant Quarantine

摘　　要：传统的线虫形态学鉴定存在经验性强和幼虫难以鉴定等不足。本文根据线虫ITS基因序列多态性 ,设计鳞球茎茎线虫 (Ditylenchusdipsaci)特异的TaqMan探针 ,建立了实时荧光PCR检测方法。对茎线虫属的鉴定实验表明 :只有鳞球茎茎线虫产生特异性荧光信号 ,同属的其他线虫均检测到荧光信号。整个检测过程只需 30min到 2h ,能够满足口岸检验检疫快速通关的要求。Stem bulb nematode(Ditylenchus dipsaci )is a dangerous and damage nematodes in the world,and is also in the A 2 li st of quarantine pests concerned to China.A real time PCR method was developed for detection of D.dipsaci.The species-specific probe for D.dipsaci was developed,and no fluorescent signal was detected fo r other nematode except D.dipsaci.The detection process only n eeds 30min or 2 hours,so the method is specific and little time consumption.

关 键 词：线虫 球茎 形态学鉴定 幼虫 TAQMAN探针 实时荧光PCR检测方法 检验检疫 特异性 多态性 基因序列 

分 类 号：S41[农业科学—植物保护]