大鼠腰骶段脊髓的脑啡肽能传入纤维的脑起源  

The origins of the L-ENK ephalinergic afferents fiber within lumbosacral spinal cord in rat

作　　者：冯广友[1] 周祥庭 

机构地区：[1]河南医科大学解剖学教研室

出　　处：《新乡医学院学报》1994年第1期24-27,共4页Journal of Xinxiang Medical University

摘　　要：研究目的探讨大鼠下丘脑和脑干至脊髓腰6和骶1节段的亮氨酸－脑啡肽能（L-ENK）下行投射纤维的细胞起源。研究设计用HRP注入大鼠腰骶髓，观察下丘脑及脑干的HRP标记细胞部位，然后用免疫细胞化学方法处理标记细胞，对其递质定性。研究对象健康成年Wistar大鼠。研究方法HRP注入腰6和骶1的一侧半后，取间脑和整个脑干冰冻切片，TMB成色，经过DAB＋COCl2法稳定1－MB产物后，再经L－ENK抗体孵育及PAP法至色，观察HRP和L－ENK免疫反应江标细胞。结果下丘脑室旁核、红核、前庭神经外侧核、Minimus核、脑桥尾测网状核．巨细胞网状核均出现了HRP－L－ENK双标细胞。结论下丘脑室旁核及脑干有脑啡肽能神经元投射至大鼠腰骶段脊髓。Objective To investigate the projection from the enkephalinergic nerons in the hypothalamus and brainstem to the lumbosacral spinal cord in rat.Metbods Six normal adult rats of Wistar strain were used. The position of HRP labeled cells was investigated after injection into the half side of the lumbosacral spinal cord, and then the labeled cells were stained with the immunocellochemical method, and whether the enkephalinergic transmitter or not were determined in the labeled cells. The sections of diencephalin and brain stem were stained with TMB method, and then TMB products were stabilised in the DAB + CoCl2 liquid. The sections were incubated in L-ENK antiserum and stained with PAP method.Results HRP-L-ENK double-labeled cells were found in paraventricular nucleus of hypothalamus, red nucleus, lateral vestibular nucleus, minimus nucleus, reticular nucleus of pontis caudalis, gigantocellular reticular nucleus.Couclutiou This study suggests that theneurons containing L-enkephalin in the hypothalamus and the beainstem are project to lumbral 6 and sacral 1 segments of sDinal cord in rat.

关 键 词：脊髓 脑干 脑啡肽 传入纤维 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]