全二维液相色谱(IEC/RP)的构建与评价  

Establishment and Evaluation of Comprehensive Two-dimensional Liquid Chromatography(IEC/RP)

作　　者：王智聪[1] 张庆合[1] 张维冰[2] 李彤 赵中一[1] 

机构地区：[1]中国地质大学材料与化学工程学院,武汉430074 [2]中国科学院大连化学物理研究所,大连116011 [3]大连依利特分析仪器有限公司,大连116011

出　　处：《高等学校化学学报》2005年第3期426-429,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 3 75 0 40 )资助

摘　　要：以 IEC/RP模式构建了二维液相色谱系统 ,采用平行交替柱捕集分析的 2位十通切换阀作接口 ,第一维洗脱产物按 1 0∶ 1的分流比分流后 ,得到 IEC和 RP切换谱图 .以 5个标准蛋白混合物的分离评价该系统 ,在单独一维模式中不能分离的样品在全二维液相色谱中得到了很好的分离 .二维系统的峰容量为 1A two-dimensional liquid chromatography is established with ion-exchange chromatography(IEC) followed by reversed phase liquid chromatography(RP). The interface is a ten-port two-position switching valve coupled with two parallel RP columns. After splitting the IEC effluent with a ratio of 10∶1(volume ratio), the IEC profile and the RP profile were obtained simultaneously. The system is evaluated with 5 standard proteins, which cannot be resolved in either of IEC or RP mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. The total peak capacity of this system is calculated to be 189.

关 键 词：全二维液相色谱 蛋白质 峰容量 

分 类 号：O658[理学—分析化学]